鞣花酸对骨肉瘤MG-63细胞的生物学作用及分子机制  

Biological effect and molecular mechanism of ellagic acid on osteosarcoma MG-63

作　　者：赵宁[1] 周新卫[1] 郑荣[1] 梁波[1] 陈劲[1] 范晓敏 ZHAO Ning;ZHOU Xin-Wei;ZHENG Rong(The Trauma Orthopedics Department,The Central People's Hospital of Zhanjiang,Zhanjiang 524000,Guangdong,China)

机构地区：[1]湛江中心人民医院创伤骨科,广东湛江524000

出　　处：《吉林医学》2024年第12期2891-2894,共4页Jilin Medical Journal

基　　金：湛江市科技攻关计划项目[项目编号:2019B01094]。

摘　　要：目的:探索天然提取物鞣花酸(EA)对骨肉瘤MG-63细胞增殖及凋亡的生物学作用和分子机制,寻求防治骨肉瘤的新途径。方法:以0μg/ml、15μg/ml、30μg/ml和45μg/ml的EA处理MG-63细胞株,通过细胞增殖实验(MTT法)和流式细胞仪检测分析EA对MG-63细胞株增殖、凋亡和细胞周期的影响,采用Western印迹检测技术观察表皮生长因子受体(EGFR),细胞增殖与细胞凋亡因子Bax和Bcl-2表达的蛋白表量变化。结果:实验组随EA浓度增加,其孵育的人骨肉瘤MG-63细胞,早期凋亡率与抑制率逐渐增高,呈现明显浓度依赖性;EA处理后的MG-63细胞阻滞于G0/G1期;EGFR表达量降低,促肿瘤细胞凋亡因子Bax蛋白表达量增加、抑肿瘤细胞凋亡因子Bcl-2蛋白表达量降低。结论:EA能够抑制MG-63细胞株增殖并诱导其凋亡,可能与EGFR、细胞因子Bax及Bcl-2表达量改变相关。Objective To explore the biological effect and molecular mechanism of natural extract ellagic acid on the proliferation and apoptosis of osteosarcoma MG-63 cells,and to seek a new way to prevent and treat osteosarcoma.Method MG-63 cell lines were treated with 0μg/ml,15μg/ml,30μg/ml,and 45μg/ml of ellagic acid,Through Methyl thiazolyl tetrazolium assayand flow cytometry,The effects of ellagic acid on proliferation,apoptosis and cell cycle of MG-63 cell line were analyzed.Western blotting was used to observe the changes of epidermal growth factor receptor(EGFR),cell proliferation and apoptosis factors Bax,Bcl-2 protein expression.Results As the concentration of ellagic acid increased in the experimental group,the early apoptosis rate and inhibition rate of human osteosarcoma MG-63 cells incubated with ellagic acid gradually increased in a concentration dependent manner;MG-63 cells treated with tannic acid were arrested in G0/G1 phase;The expression of epithelialgrowth factor receptor(EGFR)decreased,the expression of pro-tumor cell apoptosis factor Bax protein increased,and the expression of tumor apoptotic factor Bcl-2 protein decreased.Conclusion Ellagic acid can inhibit the proliferation and induce apoptosis of MG-63 cell line,which may be related to the changes in the expression of epidermal growth factor receptor(EGFR),cytokines Bax and Bcl-2.

关 键 词：鞣花酸 骨肉瘤MG-63细胞 增殖 凋亡 

分 类 号：R738.1[医药卫生—肿瘤]