岩藻黄素对高脂秀丽隐杆线虫的降脂作用及机制初探  

Lipid-lowering effect of fucoxanthin on high-fat Caenorhabditis elegans and its preliminary mechanism

作　　者：王丽瑶 陈雨欣 褚瑶 李茉琳 雷晓凌 朱国萍 卢虹玉 吴慧 WANG Liyao;CHEN Yuxin;CHU Yao;LI Molin;LEI Xiaoling;ZHU Guoping;LU Hongyu;WU Hui(College of Food Science and Technology,Guangdong Ocean University,Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety,Guangdong Marine Bioproducts Engineering Laboratory,Guangdong Marine Food Engineering Technology Research Center,Guangdong Provincial Key Laboratory of Aquatic Products Deep Processing in Common Colleges and Universities,Zhanjiang 524088,China;Collaborative Innovation Center of Key Technologies for Marine Food Deep Processing,Dalian Polytechnic University,Dalian 116034,China;School of Engineering,Guangzhou College of Technology and Business,Guangzhou 510850,China)

机构地区：[1]广东海洋大学食品科技学院,广东省水产品加工与安全重点实验室,广东省海洋生物制品工程实验室,广东省海洋食品工程技术研究中心,水产品深加工广东普通高等学校重点实验室,广东湛江524088 [2]大连工业大学海洋食品精深加工关键技术省部共建协同创新中心,辽宁大连116034 [3]广州工商学院工学院,广东广州510850

出　　处：《食品与发酵工业》2024年第23期156-161,共6页Food and Fermentation Industries

基　　金：广东海洋大学大学生创新创业训练计划项目(CXXL2023023)。

摘　　要：该研究主要探讨岩藻黄素(fucoxanthin,Fu)的降脂作用及初步机制。以50 mmol/L葡萄糖诱导建立秀丽隐杆线虫高脂肪模型,观察Fu对高脂线虫的甘油三酯含量(triglyceride,TG)、脂肪沉积、生长发育、运动行为、寿命、抗氧化酶活力的影响,进一步通过荧光实时定量PCR测定与脂肪代谢有关的关键基因转录水平。结果表明,与模型组相比,50、75和100 mg/L的Fu均能显著降低线虫的TG,减少脂肪沉积,其中100 mg/L Fu的作用最强,与空白组接近,为(0.75±0.050)mmoL/g prot,并能够改善高脂线虫的体长体宽、头部摆动频率和身体弯曲频率,高浓度Fu干预还能显著增加高脂线虫的平均寿命10.28%(P<0.05)和最长寿命(21 d);与模型组相比,不同浓度Fu处理均能够显著增加线虫体内的超氧化物歧化酶和还原性谷胱甘肽水平,最多分别提高了127.17%和265.14%(P<0.05)。在75 mg/L Fu干预后,线虫体内脂肪代谢相关关键基因daf-2、daf-16、cpt-2、mdt-15、acs-2、spb-1、tph-1、fat-5转录水平产生了显著变化(P<0.05)。因此,Fu对高脂线虫具有降脂作用,能改善高脂带来的多种不利影响,具有体内抗氧化作用,其机制涉及复杂的多个信号通路。In present study,the lipid-lowering effects of fucoxanthin(Fu)and its preliminary mechanisms were explored.The hyperadipose model of Caenorhabditis elegans was established by induction of 50 mmol/L glucose to observe the effects of Fu on triglyceride content(TG),fat deposition,growth and development,motor behavior,longevity and antioxidant enzyme activity of C.elegans.In further,the transcriptional levels of key genes related to fat metabolism were determined by fluorescent real-time quantitative PCR.Results showed that compared with the model group,50 mg/L,75 mg/L,and 100 mg/L Fu significantly reduced TG and fat deposition in C.elegans,among which 100 mg/L Fu had the strongest effect close to that of the blank group[(0.75±0.050)mmoL/g prot)].Similarly,50,75,and 100 mg/L Fu improved the body length and width,head wagging frequency and body bending frequency of high-fat C.elegans.High-concentration Fu intervention significantly increased the average life span of high-fat C.elegans by 10.28%(P<0.05)and the longest life span(21 days).Compared with the model group,superoxide dismutase(SOD)and glutathione(GSH)levels in C.elegans treated with different concentrations of Fu were significantly increased by 127.17%and 265.14%(P<0.05)respectively.Compared with the model group,different concentrations of Fu treatment significantly increased the SOD and GSH levels in C.elegans with the highest enhancements of 127.17%and 265.14%,respectively.After 75 mg/L Fu intervention,the transcription levels of key genes related to fat metabolism in C.elegans including daf-2,daf-16,cpt-2,mdt-15,acs-2,spb-1,tph-1,and FAT-5 were significantly changed(P<0.05).In conclusion,Fu has lipid-lowering effect on high-fat C.elegans,can ameliorate various adverse effects caused by hyperlipiditis,has an antioxidant effect in vivo,and its underlying mechanism involves complex signaling pathways.

关 键 词：岩藻黄素 秀丽隐杆线虫 甘油三酯 信号通路 降脂 

分 类 号：TS201.4[轻工技术与工程—食品科学]