染料脱色过氧化物酶在木质素降解中的应用研究进展  

Research progress on application of dye-decolorizing peroxidases in lignin degradation

作　　者：许艳东 杨趁仙[1] 杨寒香 高梦祥 李天赐 辛颖[1] 段晓杰 朱婷伟[1] XU Yandong;YANG Chenxian;YANG Hanxiang;GAO Mengxiang;LI Tianci;XIN Ying;DUAN Xiaojie;ZHU Tingwei(College of Food Science and Engineering,Henan University of Technology,Zhengzhou 450001,China)

机构地区：[1]河南工业大学粮油食品学院,河南郑州450001

出　　处：《食品与发酵工业》2024年第23期339-347,共9页Food and Fermentation Industries

基　　金：国家自然科学基金项目(32202079);河南省博士后科研启动项目(HN2022046);河南省科技研发计划联合基金(应用攻关类)(232103810064);河南省重点研发与推广专项(科技攻关)项目(222102110112);河南工业大学创新基金支持计划专项资助(2021ZKCJ03);河南工业大学青年骨干教师培育计划(21421237)。

摘　　要：在对玉米秸秆、小麦麸皮等植物生物质资源的开发利用过程中发现,木质素结构复杂,难以降解,极大影响了内部活性物质的释放和原料的加工特性。因此,降解木质素是提高植物生物质资源利用价值的重要步骤之一。常用的木质素降解预处理方法有物理法、化学法和生物法,生物法主要是采用微生物或其产生的生物酶对木质素进行降解,其中染料脱色过氧化物酶(dye-decolorizing peroxidases EC.1.11.1.19,DyPs)对木质素的处理具有积极作用,并能够降解不同的染料、氧化类胡萝卜素、甲氧基化芳烃等。DyPs与其他真菌过氧化物酶如木质素过氧化物酶(lignin peroxidase,LiP)、锰过氧化物酶(manganese peroxidase,MnP)存在很大差异,在氨基酸序列、蛋白质结构、催化残基、物理化学性质等方面有很大不同。因此,该文从氨基酸序列、酶学性质等方面综述了DyPs的结构和催化特性,总结了典型的DyPs对木质素的高效降解能力。同时,该文基于基因工程对提高DyPs稳定性和催化活性的研究策略以及DyPs的重要工业应用和面临的挑战进行了综述。In the development and utilization of plant biomass resources,such as corn straw and wheat bran,it is found that lignin is difficult to degrade because of its complex structure,which greatly hinders the release of internal active substances and affects the processing characteristics of raw materials.Therefore,degradation of lignin is one of the important steps to improve the utilization value of plant biomass resources.The pretreatment methods commonly used for lignin degradation are physical methods,chemical methods,and biological methods.The biological method mainly uses microorganisms or their enzymes to degrade lignin,among which the dye-decolorizing peroxidases(EC.1.11.1.19 DyPs)play a positive effect on lignin treatment and can degrade different dyes,oxidize carotenoids,and methoxylated aromatics,etc.DyPs are very different from other fungal peroxidases such as lignin peroxidase(LiP)and manganese peroxidase(MnP)in terms of amino acid sequence,protein structure,catalytic residues,physical and chemical properties.Therefore,this paper reviewed the structures and catalytic properties of DyPs in terms of amino acid sequence and enzymatic properties and summarized the efficient degradation ability of the typical DyPs on lignin.Meanwhile,the research strategies to improve the stability and catalytic activity of DyPs based on genetic engineering as well as the important industrial applications and challenges of DyPs were reviewed in this paper.

关 键 词：染料脱色过氧化物酶 木质素 降解机理 基因改造 

分 类 号：TQ426.97[化学工程] O636.2[理学—高分子化学]