玉米抗莠去津相关性状的全基因组关联分析及候选基因预测  

Genome-Wide Association Analysis of Atrazine Resistance Related Traits and Candidate Gene Prediction in Maize

作　　者：肖平方 肖逸飞 吴舟 黄一 陈宝 涂亮 刘鹏飞 蒋喻林 郭向阳[2] 王安贵[2] 祝云芳[2] 陈泽辉[2] 吴迅 Xiao Pingfang;Xiao Yifei;Wu Zhou;Huang Yi;Chen Bao;Tu Liang;Liu Pengfei;Jiang Yulin;Guo Xiangyang;Wang Angui;Zhu Yunfang;Chen Zehui;Wu Xun(Agricultural College,Guizhou University,Guiyang 550025,China;Institute of Upland Food Crops,Guizhou Academy of Agricultural Sciences,Guiyang 550006,China;College of Life Sciences,Guizhou University,Guiyang 550025,China;Key Laboratory of Crop Genetic Resources and Germplasm Innovation in Karst Region,Ministry of Agriculture and Rural Affairs,Guiyang 550006,China)

机构地区：[1]贵州大学农学院,贵州贵阳550025 [2]贵州省农业科学院旱粮研究所,贵州贵阳550006 [3]贵州大学生命科学学院,贵州贵阳550025 [4]农业农村部喀斯特山区作物基因资源与种质创新重点实验室,贵州贵阳550006

出　　处：《山东农业科学》2024年第11期28-35,共8页Shandong Agricultural Sciences

基　　金：国家重点研发计划项目(2023YFD1201103);贵州省科技支撑计划项目(黔科合支撑〔2022〕重点029);贵州省山地农业关键核心技术攻关项目(GZNYGJHX-2023012);贵州省育种科研基础平台创新能力建设项目(黔科合服企〔2022〕014);黔农科院国基后补助(〔2021〕16号,〔2022〕02号,〔2022〕09号);贵州喀斯特山区重要作物生物育种平台建设项目(黔科合中引地〔2023〕033);贵州省科研机构创新能力建设专项(〔2022〕007)。

摘　　要：为挖掘玉米抗除草剂相关候选基因,本研究以包含234份玉米自交系的关联群体为材料,结合包含56000个SNPs标记的基因型鉴定结果和该群体抗莠去津相关性状的评价数据进行全基因组关联分析。结果显示,全基因组关联分析共鉴定出62个与玉米抗莠去津相关性状显著关联的数量性状遗传位点(QTNs)(P<0.001),其中PZE-104041162和PZE-104041163同时与叶绿素含量和玉米受害率两个性状相关。候选基因分析共鉴定出57个玉米抗莠去津相关候选基因,其中GRMZM2G311465、GRMZM2G017536、GRMZM2G172826、GRMZM2G129431、GRMZM2G334791、GRMZM2G177878、GRMZM2G030768等基因主要参与植物生长发育、植物免疫、植物激素信号传导的调节,生物胁迫以及非生物胁迫响应过程等。研究结果为进一步深探讨不同玉米种质对莠去津的抗性机制提供关键候选基因。To identify candidate genes related to herbicide resistance in maize,this study was conducted by using an association population comprising 234 inbred lines of maize as material.The genome-wide associa-tion analysis(GWAS)was conducted through integrating the genotyping results with 56000 SNP markers and evaluation data of the traits related to atrazine resistance.The results indicated that a total of 62 quantitative trait nucleotides(QTNs)significantly(P<0.001)associated with maize resistance to atrazine were identified.Among them,PZE-104041162 and PZE-104041163 were associated with both chlorophyll content and maize injury rate.Through candidate gene analysis,57 candidate genes linked to maize resistance to atrazine were i-dentified;among which,GRMZM2G311465,GRMZM2G017536,GRMZM2G172826,GRMZM2G129431,GRMZM2G334791,GRMZM2G177878 and GRMZM2G030768 were primarily involved in regulating plant growth and development,immunity,hormonal signaling pathways,and responses to biotic and abiotic stresses.These findings could provide key candidate genes for further exploration of the resistance mechanisms to at-razine in different maize germplasms.

关 键 词：玉米 莠去津 全基因组关联分析 候选基因 分子标记 

分 类 号：S513[农业科学—作物学] Q78[生物学—分子生物学]