Catalytically active prokaryotic Argonautes employ phospholipase D family proteins to strengthen immunity against different genetic invaders  

作　　者：Feiyue Cheng Aici Wu Zhihua Li Jing Xu Xifeng Cao Haiying Yu Zhenquan Liu Rui Wang Wenyuan Han Hua Xiang Ming Li 

机构地区：[1]Department of Microbial Physiological&Metabolic Engineering,State Key Laboratory of Microbial Resources,Institute of Microbiology,Chinese Academy of Sciences,Beijing,China [2]College of Life Science,University of Chinese Academy of Sciences,Beijing,China [3]State Key Laboratory of Microbial Resources,Institute of Microbiology,Chinese Academy of Sciences,Beijing,China [4]State Key Laboratory of Agricultural Microbiology and College of Life Science and Technology,Hubei Hongshan Laboratory,Huazhong Agricultural University,Wuhan,China

出　　处：《mLife》2024年第3期403-416,共14页微生物（英文）

基　　金：This work was supported by the Science&Technology Fundamental Resources Investigation Program(2022FY101100);the Strategic Priority Research Program of the Chinese Academy of Sciences(Precision seed design and breeding)(XDA24020101);the National Natural Science Foundation of China(Nos.31970544,31970545,32022003,32150020,32200057,and 32270092);the Youth Innovation Promotion Association of CAS(No.2020090);the China National Postdoctoral Program for Innovative Talents(BX20220331);the China Postdoctoral Science Foundation(2022M720160);the Special Research Assistant Program of Chinese Academy of Sciences(No.2023000056).

摘　　要：Prokaryotic Argonautes(pAgos)provide bacteria and archaea with immunity against plasmids and viruses.Catalytically active pAgos utilize short oligonucleotides as guides to directly cleave foreign nucleic acids,while inactive pAgos lacking catalytic residues employ auxiliary effectors,such as nonspecific nucleases,to trigger abortive infection upon detection of foreign nucleic acids.Here,we report a unique group of catalytically active pAgo proteins that frequently associate with a phos-pholipase D(PLD)family protein.We demonstrate that this particular system employs the catalytic center of the associated PLD protein rather than that of pAgo to restrict plasmid DNA,while interestingly,its immunity against a single-stranded DNA virus relies on the pAgo catalytic center and is enhanced by the PLD protein.We also find that this system selectively suppresses viral DNA propagation without inducing noticeable abortive infection outcomes.Moreover,the pAgo protein alone enhances gene editing,which is unexpectedly inhibited by the PLD protein.Our data highlight the ability of catalytically active pAgo proteins to employ auxiliary proteins to strengthen the targeted eradication of different genetic invaders and underline the trend of PLD nucleases to participate in host immunity.

关 键 词：ARGONAUTE DNA interference genome editing phage defense PLD protein 

分 类 号：Q93[生物学—微生物学]