This work was supported by the Science&Technology Fundamental Resources Investigation Program(2022FY101100);the Strategic Priority Research Program of the Chinese Academy of Sciences(Precision seed design and breeding)(XDA24020101);the National Natural Science Foundation of China(Nos.31970544,31970545,32022003,32150020,32200057,and 32270092);the Youth Innovation Promotion Association of CAS(No.2020090);the China National Postdoctoral Program for Innovative Talents(BX20220331);the China Postdoctoral Science Foundation(2022M720160);the Special Research Assistant Program of Chinese Academy of Sciences(No.2023000056).
Prokaryotic Argonautes(pAgos)provide bacteria and archaea with immunity against plasmids and viruses.Catalytically active pAgos utilize short oligonucleotides as guides to directly cleave foreign nucleic acids,while i...
supported by the grants from the China Postdoctoral Science Foundation(No.2022M721665);the Research Program of the"521 Project"of Lianyungang City of China(No.LYG06521202133);the Lianyungang Key R&D Project(No.CG2305);the Lianyungang Postdoctoral Research Funding Program(No.LYG20220022);the Open-end Funds of Jiangsu Key Laboratory of Marine Bioresources and Environment(Nos.SH20221208 and SH20231212);the"Blue Project"of Jiangsu Higher Education Institutions of China;the Priority Academic Program Development of Jiangsu Higher Education Institutions of China.
The leucine zipper transcription factor-like 1(LZTFL1)gene is active in respiratory and lung epithelia.Mutation of base G to A at the rs17713054 locus on the LZTFL1 allele causes an increase in the expression of the g...
supported by the Ministry of Science and Technology(2020YFA0907700);National Natural Science Foundation of China(31770078,32270051);Shanghai Pilot Program for Basic Research-Shanghai Jiao Tong University(21TQ1400204).
Mesophilic Argonautes(Agos)from microbial resources have received significant attention due to their potential applications in genome editing and molecular diagnostics.This study characterizes a novel Ago from Pseudob...
supported by the National Key R&D Program of China(2022YFA1302900 to B.W.);the National Natural Science Foundation of China(31825010 and 82121005 to B.W.);CAS Strategic Priority Research Program(XDB37030100 to B.W.and Q.Z.);Shanghai Pilot Program for BasicResearch Chinese Academy of Sciences,Shanghai Branch(JCYJ-SHFY-2021-008 to B.W.);the Shanghai Science and Technology Committee grant(23YF1456600 to S.L.).
Nicotinamide adenine dinucleotide(NAD+)is a central metabolite in cellular processes.Depletion of NAD+has been demonstrated to be a prevalent theme in both prokaryotic and eukaryotic immune responses.Short prokaryotic...
Unusual Argonaute(Ago)proteins have been recently identified in prokaryotic immune systems,which upon binding to foreign nucleic acids,activate their associated NADase effectors.Recent cryo-EM structures of such uniqu...