ARGONAUTE

作品数:94被引量:242H指数:8
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相关领域:生物学医药卫生更多>>
相关作者:王飞翟立红郜恒骏潘志雄唐慧更多>>
相关机构:中南财经政法大学华中农业大学浙江大学四川农业大学更多>>
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相关基金:国家自然科学基金国家重点基础研究发展计划国家高技术研究发展计划国家教育部博士点基金更多>>
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Catalytically active prokaryotic Argonautes employ phospholipase D family proteins to strengthen immunity against different genetic invaders
《mLife》2024年第3期403-416,共14页Feiyue Cheng Aici Wu Zhihua Li Jing Xu Xifeng Cao Haiying Yu Zhenquan Liu Rui Wang Wenyuan Han Hua Xiang Ming Li 
This work was supported by the Science&Technology Fundamental Resources Investigation Program(2022FY101100);the Strategic Priority Research Program of the Chinese Academy of Sciences(Precision seed design and breeding)(XDA24020101);the National Natural Science Foundation of China(Nos.31970544,31970545,32022003,32150020,32200057,and 32270092);the Youth Innovation Promotion Association of CAS(No.2020090);the China National Postdoctoral Program for Innovative Talents(BX20220331);the China Postdoctoral Science Foundation(2022M720160);the Special Research Assistant Program of Chinese Academy of Sciences(No.2023000056).
Prokaryotic Argonautes(pAgos)provide bacteria and archaea with immunity against plasmids and viruses.Catalytically active pAgos utilize short oligonucleotides as guides to directly cleave foreign nucleic acids,while i...
关键词:ARGONAUTE DNA interference genome editing phage defense PLD protein 
原核Argonaute的应用研究进展
《华中农业大学学报》2024年第4期82-93,共12页王飞 李文强 刘洋 王珑瑜 陈晚苹 崔佳凯 马立新 
国家重点研发计划项目(2021YFC2100100);湖北省自然科学基金杰出青年项目(2024AFA101);中国博士后科学基金面上项目(2023M741100)。
原核生物Argonautes(pAgos)是参与细胞防御外来DNA入侵的可编程核酸酶。在体外,pAgos可以结合小的单链核酸(ssDNA/ssRNA)向导来识别和切割互补DNA/RNA。在体内,pAgos优先靶向多拷贝遗传元件、噬菌体和质粒,从而抑制入侵核酸的扩增和噬...
关键词:可编程核酸酶 原核Argonaute 基因编辑 分子诊断 DNA组装 
Highly sensitive detection of a long-COVID-related SNP in LZTFL1 allele with Pyrococcus furiosus Argonaute in point-of-care settings
《Acta Biochimica et Biophysica Sinica》2024年第7期1089-1092,共4页Yixin Tang Miao Xu Bo Luo Yue Wang Yukang Chen Guangxi Yu Guang Yang Song Gao Pei Wang 
supported by the grants from the China Postdoctoral Science Foundation(No.2022M721665);the Research Program of the"521 Project"of Lianyungang City of China(No.LYG06521202133);the Lianyungang Key R&D Project(No.CG2305);the Lianyungang Postdoctoral Research Funding Program(No.LYG20220022);the Open-end Funds of Jiangsu Key Laboratory of Marine Bioresources and Environment(Nos.SH20221208 and SH20231212);the"Blue Project"of Jiangsu Higher Education Institutions of China;the Priority Academic Program Development of Jiangsu Higher Education Institutions of China.
The leucine zipper transcription factor-like 1(LZTFL1)gene is active in respiratory and lung epithelia.Mutation of base G to A at the rs17713054 locus on the LZTFL1 allele causes an increase in the expression of the g...
关键词:LZTFL1 ALLELE LUNG 
Characterization of argonaute nucleases from mesophilic bacteria Pseudobutyrivibrio ruminis
《Bioresources and Bioprocessing》2024年第1期1244-1256,共13页Xiaoyi Xu Hao Yang Huarong Dong Xiao Li Qian Liu Yan Feng 
supported by the Ministry of Science and Technology(2020YFA0907700);National Natural Science Foundation of China(31770078,32270051);Shanghai Pilot Program for Basic Research-Shanghai Jiao Tong University(21TQ1400204).
Mesophilic Argonautes(Agos)from microbial resources have received significant attention due to their potential applications in genome editing and molecular diagnostics.This study characterizes a novel Ago from Pseudob...
关键词:Pseudobutyrivibrio ruminis Argonaute Mesophilic Argonaute Prokaryotic Argonaute ENDONUCLEASE DNA cleavage 
Argonaute 2和微小RNA-145-5p对肝癌细胞增殖和转移的影响
《中华实验外科杂志》2023年第12期2491-2494,共4页李威威 赵杰 
目的探讨Ago2和微小RNA(miRNA,miR)-145-5p对肝癌细胞增殖和转移的影响。方法选取2018年6月至2022年12月商丘市第一人民医院收治的98例肝癌患者肿瘤组织和癌旁组织作为研究对象,采用荧光定量聚合酶链反应(PCR)和蛋白质免疫印迹分析miR-1...
关键词:微小RNA-145-5p Ago2 肝癌 增殖 转移 
Structural insights into dimerization and activation of the mGlu2-mGlu3 and mGlu2-mGlu4 heterodimers被引量:1
《Cell Research》2023年第10期762-774,共13页Xinwei Wang Mu Wang Tuo Xu Ye Feng Qiang Shao Shuo Han Xiaojing Chu Yechun Xu Shuling Lin Qiang Zhao Beili Wu 
supported by the National Key R&D Program of China(2022YFA1302900 to B.W.);the National Natural Science Foundation of China(31825010 and 82121005 to B.W.);CAS Strategic Priority Research Program(XDB37030100 to B.W.and Q.Z.);Shanghai Pilot Program for BasicResearch Chinese Academy of Sciences,Shanghai Branch(JCYJ-SHFY-2021-008 to B.W.);the Shanghai Science and Technology Committee grant(23YF1456600 to S.L.).
Nicotinamide adenine dinucleotide(NAD+)is a central metabolite in cellular processes.Depletion of NAD+has been demonstrated to be a prevalent theme in both prokaryotic and eukaryotic immune responses.Short prokaryotic...
关键词:ARGONAUTE ACTIVATION IMMUNITY 
基于Argonaute的病毒核酸检测技术研究进展被引量:3
《病毒学报》2023年第5期1414-1424,共11页刘妍 孙凯 田春雨 马宁 付康 俞晓平 申屠旭萍 
国家重点研发课题(项目号:2021YFF0602801),题目:口岸现场核酸快速检测装备及技术研发;浙江省科技计划项目(项目号:2022C02047),题目:水稻重要病虫害绿色防控关键技术——水稻重要病虫害绿色防控关键技术研究及应用~~。
病毒性传染病对人类社会构成严重威胁,特别是近年来由新型冠状病毒SARS-CoV-2引起的冠状病毒肺炎(COVID-19)暴发,引发了全球大流行,严重危害人类生命健康。快速、敏感、便携的病毒鉴定技术对于疾病监测、控制和治疗至关重要。近年来,以c...
关键词:ARGONAUTE 病毒检测 核酸检测 核酸酶 快速检测 
神经胶质调节平行记忆形成雪旺细胞中Argonaute 2的消融加速糖尿病周围神经病变的进展被引量:1
《神经损伤与功能重建》2023年第10期F0003-F0003,共1页Baoyan Fan Michael Chopp Yi Zhang XinliWang Amy Kemper Zheng Gang Zhang Xian Shuang Liu 唐颖馨 
施万细胞(SCs)形成髓鞘并为轴突提供代谢支持,这对于正常的神经功能至关重要。鉴定出特定于SCs和神经纤维的关键分子可能为糖尿病周围神经病变(DPN)提供新的治疗目标。Argonaute2(Ago2)是介导miRNA-guided mRNA剪切和miRNA稳定性的关键...
关键词:Argonaute2 施万细胞 糖尿病周围神经病变 微小RNA 线粒体 
Atypical bacterial Argonautes regulate antiphage defense
《Cell Research》2023年第9期655-656,共2页Arpita Chakravarti Dinshaw J.Patel 
Unusual Argonaute(Ago)proteins have been recently identified in prokaryotic immune systems,which upon binding to foreign nucleic acids,activate their associated NADase effectors.Recent cryo-EM structures of such uniqu...
关键词:SIR2 ARGONAUTE activation 
猕猴桃DCL、RDR和AGO基因家族的鉴定与分析
《西北农业学报》2023年第9期1394-1403,共10页方远鹏 李云洲 赵志博 樊荣 龙友华 
贵州大学培育项目(贵大培育[2019]52号);贵州大学人才引进科研项目(贵大人基合字[2017]50号);贵州省科技支撑计划项目(黔科合支撑[2020]1Y131号);贵州省省级科技计划项目(黔科合基础-2K[2022]一般071)~~。
为研究猕猴桃RNAi抗病毒机制相关基因DCL、RDR和AGO。本研究采用blastp比对法和NPS@、SignalP-5.0及TMHMM 2.0等数据库对猕猴桃RNAi相关蛋白家族进行鉴定及其对应基础性质(如:亚细胞定位、二级结构等)的分析。结果在猕猴桃中共鉴定获得4...
关键词:RNA干扰(RNAI) Argonaute(AGO) Dicer类蛋白(DCL) RNA依赖型RNA聚合酶(RDR) 病毒 猕猴桃 
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