基于斑马鱼模型和体外抗氧化评价甘南牦牛乳抗氧化效果  

Antioxidant effect of Gannan yak milk based on zebrafish model and antioxidant in vitro

作　　者：罗丽 宋礼 张晓梅 LUO Li;SONG Li;ZHANG Xiaomei(Gansu Hualing Dairy Co.Ltd.,Gannan 747000,China;Gannan Yak Milk Research Institute,Gannan 747000,China)

机构地区：[1]甘肃华羚乳品股份有限公司,甘肃甘南747000 [2]甘南州牦牛乳研究院,甘肃甘南747000

出　　处：《食品与发酵工业》2024年第24期133-138,共6页Food and Fermentation Industries

基　　金：甘肃省青年科技基金计划项目(22 JR5RP1025);企业研发机构能力建设专项(22CX8 JP250);中央引导地方科技发展资金项目(23ZYQP0001)。

摘　　要：牦牛乳作为青藏高原特色乳资源,功能性产品研究与转化较少,不利于地方经济的发展。为探究甘南牦牛乳的抗氧化活性,该实验选取羟自由基、超氧阴离子自由基、DPPH自由基开展牦牛乳体外抗氧化效果评价;优选受精后3 dpf的斑马鱼,在不同添加量(12.5、25.0、50.0μL/mL)的牦牛乳中暴露到24 h,通过荧光染色检测斑马鱼活性氧(reactive oxygen species,ROS)产生率,分析超氧化物歧化酶活力,进行牦牛乳体内抗氧化效果评价。结果表明,牦牛乳具有清除羟自由基、DPPH自由基以及超氧阴离子自由基的能力;12.5、25.0、50.0μL/mL添加量的牦牛乳能降低斑马鱼体内ROS产生率,提高斑马鱼抗氧化酶活力,说明牦牛乳具有抗氧化活性,且牦牛乳体内抗氧化效果与其质量浓度呈正相关,当牦牛乳添加量为50.0μL/mL,对斑马鱼的抗氧化效果达到43.25%。实验表明,甘南牦牛乳具有抗氧化活性,可作为抗氧化功能性食品的原料开发。As a characteristic milk resource of the Qinghai-Tibet Plateau,yak milk was less researched and transformed into functional products,which was not conducive to the development of the local economy.To investigate the antioxidant activity of Gannan yak milk,hydroxyl radicals,superoxide anions,and DPPH radicals were selected to evaluate the antioxidant effect of yak milk in vitro,and zebrafish with 3 dpf after fertilization were exposed to yak milk with different additions(12.5,25.0,50.0μL/mL)for 24 h.The production rate of reactive oxygen species(ROS)and the activity of superoxide dismutase in zebrafish were detected by fluorescent staining,and the antioxidant effect in yak milk was evaluated.Results showed that yak milk had the ability to scavenge hydroxyl free radicals,DPPH free radicals,and superoxide anions.12.5μL/mL,25.0μL/mL,and 50.0μL/mL addition of yak milk could reduce ROS production rate and increase antioxidant oxidase activity in zebrafish,which indicated that yak milk has antioxidant activity,and the antioxidant effect in yak milk was positively correlated with its mass concentration.When the addition of yak milk was 50.0μL/mL,the antioxidant effect on zebrafish reached 43.25%.Results showed that Gannan yak milk had antioxidant activity and could be used as a raw material for antioxidant functional food.

关 键 词：甘南牦牛乳 斑马鱼 体内抗氧化 体外抗氧化 

分 类 号：TS252.1[轻工技术与工程—农产品加工及贮藏工程]