富硒黄山贡菊多糖的超滤分离及其生物活性研究  

Study on Ultrafiltration Separation and Biological Activity of Polysaccharides from Selenium‑enriched Huangshan Gongju

作　　者：李可 杜冬生 钟鸿姣 陈润泽 普艺 杭华 Li Ke;Du Dongsheng;Zhong Hongjiao;Chen Runze;Pu Yi;Hang Hua(College of Life Sciences,Anhui Normal University,Wuhu 241000,China)

机构地区：[1]安徽师范大学生命科学学院,安徽芜湖241000

出　　处：《中国野生植物资源》2024年第11期44-50,56,共8页Chinese Wild Plant Resources

基　　金：安徽省自然科学基金项目(1908085MC99);安徽省大学生创新创业训练项目(2022063822)。

摘　　要：目的:探究超滤膜分离对富硒黄山贡菊多糖的影响及不同分子量组分多糖的抗氧化和抗炎活性差异。方法:采用超滤法将富硒黄山贡菊多糖分为100 kDa+多糖和100 kDa-多糖两组分,利用抗氧化实验(羟自由基清除能力、DPPH清除能力和总还原力)和细胞炎症实验(NO试剂盒和RT-qPCR法)检测超滤前后多糖的抗氧化能力和抗炎症活性。结果:未超滤多糖(粗多糖)和100 kDa-多糖抗氧化活性优于100 kDa+多糖;粗多糖与超滤处理多糖均对IL-1β、IL-6和TNF-α分泌具有抑制效果,多糖浓度为50µg/mL时,超滤后多糖组分对IL-6和TNF-α分泌的抑制效果更为显著。结论:超滤后100 kDa-多糖具有显著的抗氧化活性,100 kDa+多糖能显著减少LPS诱导巨噬细胞的炎症反应。Objective:To investigate the effects of ultrafiltration membrane separation on the selenium-enriched Huangshan Gongju polysaccharides and the variations in the anti-oxidant and anti-inflammatory activities of different molecular weight fractions of polysaccharides.Methods:Ultrafiltration was employed to fractionate the polysaccharides extracted from selenium-enriched Huangshan Gongju,which were divided into two components of 100 kDa+polysaccharides and 100 kDa-polysaccharides.The antioxidant trials(hydroxyl radical scavenging capacity,DPPH scavenging capacity and total reducing capacity)and cell inflammation tests(NO detection kit and RT-qPCR methodology)were utilized to detect the antioxidant capacities and anti-inflammatory activities of polysaccharides obtained from before and after ultrafiltration.Results:The antioxidant activities of non-ultrafiltration polysaccharides(crude polysaccharide)and 100 kDa-polysaccharides were better than those of 100 kDa+polysaccharides.Both crude polysaccharides and ultrafiltration-treated polysaccharides had impacts on the inhibitory effects of IL-1β,IL-6 and TNF-αsecretions.When polysaccharides concentration was 50µg/mL,ultrafiltration-treated polysaccharides had better inhibitory effects on the IL-6 and TNF-αsecretions.Conclusion:The 100 kDa-polysaccharides fraction demonstrated significant antioxidant activity.The 100 kDa+polysaccharides fraction could significantly reduce inflammatory responses induced by LPS in macrophages.

关 键 词：富硒黄山贡菊 多糖 超滤 抗氧化 抗炎症 

分 类 号：TS201.1[轻工技术与工程—食品科学]