丙二酰基人参皂苷对溃疡性结肠炎小鼠的保护作用及其机制  

Protective effect of malonyl ginsenoside against ulcerative colitis in mice and its mechanism

作　　者：李嘉星 王佳美 李丹 李美慧 靳晓曼 刘志[1] LI Jiaxing;WANG Jiamei;LI Dan;LI Meihui;JIN Xiaoman;LIU Zhi(College of Chinese Medicinal Materials,Jilin Agricultural University,Changchun 130118)

机构地区：[1]吉林农业大学中药材学院,长春130118

出　　处：《中国食品添加剂》2024年第12期46-54,共9页China Food Additives

基　　金：国家自然科学基金(NO.31770378)。

摘　　要：采用葡聚糖硫酸钠(DSS)建立溃疡性结肠炎(UC)模型,通过丙二酰基人参皂苷(MGR)给药干预,检测UC小鼠血清和结肠组织中的生化指标和PI3K/AKT/NF-κB信号通路的表达,并分析小鼠肠道菌群的变化。结果显示MGR显著降低血清中IL-6、IL-1β和TNF-α水平,抑制结肠组织中MDA和MPO水平的升高以及SOD水平的降低;同时,MGR降低了p-PI3K、p-AKT、TLR4和NF-κB的表达。此外,MGR调节了肠道菌群的组成和多样性,显著降低了有害菌如脱硫弧菌、副沙门氏菌、螺杆菌的相对丰度,增加了益生菌如普雷沃氏菌和乳酸杆菌的相对丰度。综上所述,MGR可能通过抑制炎症、氧化应激和调节肠道菌群失衡发挥抗UC作用。A ulcerative colitis(UC)model was established using dextran sulfate sodium(DSS),and intervention with Malonyl Ginsenosides(MGR)was employed.Biochemical markers in serum and colonic tissues of UC mice were measured,along with the expression of the PI3K/AKT/NF-κB signaling pathway in UC mice,and the alteration in intestinal microbiota was analyzed.Results revealed that MGR significantly decreased levels of IL-6,IL-1β,and TNF-αin serum,inhibited the elevation of MDA and MPO levels,and restored SOD levels in colonic tissues.Additionally,MGR downregulated the expression of p-PI3K,p-AKT,TLR4,and NF-κB.Furthermore,MGR modulated the composition and diversity of intestinal microbiota,reduced the relative abundance of harmful bacteria such as Desulfovibrionaceae,Parasutterella,and Helicobacter,while increased that of beneficial bacteria like Prevotellaccae and Lactobacillus.In conclusion,MGR may exert its anti-UC effects by suppressing inflammation,oxidative stress,and rebalancing intestinal microbiota.

关 键 词：丙二酰基人参皂苷 溃疡性结肠炎 机制 肠道菌群 

分 类 号：TS202.3[轻工技术与工程—食品科学] S567.51[轻工技术与工程—食品科学与工程]