超高效合相色谱法测定保健食品中维生素D3含量  

Determination of vitamin D3 content in health foods using ultra performance convergence chromatography

作　　者：刘春丽 陈汉峰 杨静 卢迪勋 候少平 黎俊威 吴韶敏 宋利军 LIU Chunli;CHEN Hanfeng;YANG Jing;LU Dixun;HOU Shaoping;LI Junwei;WU Shaomin;SONG Lijun(Jietong(Guangzhou)Testing Technology Service Co.,Ltd.,Guangzhou 510730;Amway(China)Co.,Ltd.,Guangzhou 510730)

机构地区：[1]捷通(广州)检测技术服务有限公司,广州510730 [2]安利(中国)日用品有限公司,广州510730

出　　处：《中国食品添加剂》2024年第12期224-230,共7页China Food Additives

摘　　要：建立样品经DMSO预处理,正己烷提取,氮吹仪吹干后,再用2m L甲醇乙腈复溶溶剂,溶解后用超高效合相色谱(Ultra Performance Convergence Chromatography UPCC)分析测定保健食品中维生素D3含量的方法,实现维生素D3在1.0~11μg/m L范围内线性关系良好,相关系数R为1.0000,回收率均大于85%(n=9),相对标准偏差值为3.11%~4.82%,符合相关国标要求。本方法检出限为0.5 mg/kg,定量限为1.5mg/kg,简单、快速、稳定,有效解决了传统高效液相色谱法样品处理复杂、检测周期长、成本高等问题。A method for the determination of vitamin D3 content in health foods was established,the samples were pretreated with DMSO,extracted with n-hexane,dried with a nitrogen blow dryer,and samples were re-dissolved in 2 mL of a methanol-acetonitrile solvent mixture and analyzed using ultra performance convergence chromatography(UPCC).The method demonstrated good linearity for vitamin D3 in the range of 1.0 to 11μg/mL,with a correlation coefficient(R)of 1.0000.Recovery rates were greater than 85%(n=9),and the relative standard deviation was 3.11%〜4.82%,meeting the relevant national standards.The limit of detection was 0.5 mg/kg,the limit of quantification was 1.5 mg/kg.This method was simple,rapid and stable,effectively addressing the challenges of traditional high-performance liquid chromatography,such as complex sample processing,lengthy detection periods,and high costs.

关 键 词：超高效合相色谱法 维生素D3 保健食品 

分 类 号：TS218[轻工技术与工程—粮食、油脂及植物蛋白工程] Q56[轻工技术与工程—食品科学与工程]