L-苏糖酸镁对非小细胞肺癌H460细胞迁移和侵袭能力及NF-κB/TNF-α信号通路的影响  

Effects of magnesium-L-threonate on migration and invasion ability and NF-κB/TNF-alpha signaling pathway of non-small cell lung cancer H460 cells

作　　者：熊媖 沈静娇 刘先国[3] 周鑫 XIONG Ying;SHEN Jingjiao;LIU Xianguo;ZHOU Xin(Department of Anesthesiology,Guangdong Provincial People's Hospital(Guangdong Academy of Medical Sciences),Southern Medical University,Guangdong Guangzhou 510000,China;Affiliated South China Hospital,Southern Medical University(Guangdong Provincial People's Hospital),Guangdong Guangzhou 510000,China;Sun Yat-sen Medical College,Sun Yat-sen University,Guangdong Guangzhou 510000,China;Guangdong Cardiovascular Institute,Guangdong Provincial People's Hospital,Guangdong Academy of Medical Sciences,Guangdong Guangzhou 510000,China)

机构地区：[1]南方医科大学附属广东省人民医院(广东省医学科学院)麻醉科,广东广州510000 [2]南方医科大学附属华南医院(广东省人民医院),广东广州510000 [3]中山大学中山医学院,广东广州510000 [4]广东省心血管病研究所,广东省人民医院,广东省医学科学院,广东广州510000

出　　处：《现代肿瘤医学》2024年第24期4580-4587,共8页Journal of Modern Oncology

基　　金：中国博士后科学基金(编号:2020M682641);广东省基础与应用基础研究基金(编号:2020A1515110959,2021A1515111090)。

摘　　要：目的:探讨L-苏糖酸镁(magnesium-L-threonate,L-TAMS)对非小细胞肺癌H460细胞迁移和侵袭能力的影响及机制。方法:正常永生肺上皮细胞16HBE和非小细胞肺癌细胞株H460培养液中加入L-TAMS或硫酸镁(MgSO_(4))共培养;流式细胞术检测细胞内镁离子浓度变化,划痕实验检测细胞迁移能力,Transwell实验检测细胞侵袭能力,Western blot、免疫荧光染色(IF)及RT-qPCR检测NF-κB/TNF-α通路相关蛋白表达变化。结果:流式细胞术结果显示,相对于对照组16HBE细胞,H460细胞内游离镁离子浓度降低,而0.4 mmol/L L-TAMS可以补充其细胞内游离镁离子;划痕实验结果显示0.4 mmol/L L-TAMS可以显著抑制H460细胞迁移能力,且效果优于0.4 mmol/L MgSO_(4);Transwell侵袭实验显示L-TAMS可抑制H460细胞侵袭能力;Western blot、IF、RT-qPCR实验发现L-TAMS可以时间依赖性抑制NF-κB/TNF-α通路,并在24 h时0.8 mmol/L L-TAMS效果优于0.4 mmol/L L-TAMS。结论:L-TAMS能抑制非小细胞肺癌H460细胞的侵袭和迁移能力并同时抑制NF-κB/TNF-α通路,本研究为临床上寻找预防非小细胞肺癌细胞药物提供了新思路。Objective:To investigate the effects and mechanism of magnesium-L-threonate(L-TAMS)on the migration and invasion of non-small cell lung cancer H460 cells.Methods:Normal immortal lung epithelial cells 16HBE and non-small cell lung cancer cell line H460 were co-cultured with L-TAMS or magnesium sulfate(MgSO_(4)).Intracellular magnesium concentration was detected by flow cytometry,cell migration ability was detected by scratches assay,cell invasion ability was detected by Transwell assay,and the expression of NF-κB/TNF-α pathway-related proteins was detected by Western blot,immunofluorescence staining(IF)and RT-qPCR.Results:Flow cytometry showed that the concentration of free magnesium ions in H460 cells decreased compared with that in the control group,and 0.4 mmol/L L-TAMS could supplement the concentration of free magnesium ions in H460 cells.Scratch test results showed that 0.4 mmol/L L-TAMS could significantly inhibit the migration of H460 cells,and the effect was better than that of 0.4 mmol/L MgSO_(4).The Transwell invasion experiment showed that L-TAMS inhibited the invasion ability of H460 cells.Western blot,IF,and RT-qPCR showed that L-TAMS could inhibit NF-κB/TNF-α pathway in a time-dependent manner,and the effect of 0.8 mmol/L L-TAMS was better than that of 0.4 mmol/L L-TAMS at 24 h.Conclusion:L-TAMS inhibited the invasion and migration and the NF-κB/TNF-α pathway of non-small cell lung cancer H460 cells,which provides a new way to search for cell drugs to prevent NSCLC in the clinic.

关 键 词：L-苏糖酸镁 非小细胞肺癌 迁移 侵袭 NF-κB/TNF-α信号通路 

分 类 号：R734.2[医药卫生—肿瘤]