食管鳞状细胞癌组织和细胞中miR-1的表达及其与患者临床病理特征的相关性  

Expression of miR-1 in tissues and cells of esophageal squamous cell carcinoma and its corrlation with clinicopathological features of patients

作　　者：姚丽 左伟晨 苟思琪 程钲益 王娟红 YAO Li;ZUO Weichen;GOU Siqi;CHENG Zhengyi;WANG Juanhong(Department of Pathology,Xi'an No.3 Hospital,the Affiliated Hospital of Northwest University,Shaanxi Xi'an 710018,China)

机构地区：[1]西北大学附属医院·西安市第三医院病理科,陕西西安710018

出　　处：《现代肿瘤医学》2024年第23期4459-4462,共4页Journal of Modern Oncology

基　　金：陕西省重点研发计划项目(编号:2024SF-YBXM-115)。

摘　　要：目的:检测miR-1在食管鳞状细胞癌(esophageal squamous cell carcinoma,ESCC)中的表达情况及其与临床病理特征的相关性。方法:64例食管鳞状细胞癌组织标本及5种食管鳞状细胞癌细胞系,通过反转录PCR和实时定量荧光PCR技术检测miR-1表达情况,应用统计软件SPSS,采用t检验及ANOVA检验统计学分析miR-1的表达水平差异及与临床病理特征相关性。结果:90.6%的患者食管鳞状细胞癌组织中miR-1的表达较正常组织下调倍数大于2倍。miR-1在5种食管鳞状细胞癌细胞系中表达量都显著降低。统计学分析发现miR-1的表达水平与食管鳞状细胞癌肿瘤浸润深度及临床分期显著相关,与患者性别、年龄、肿瘤大小以及分化程度无明显相关性。结论:miR-1在食管鳞状细胞癌中表达下调并且与肿瘤浸润深度及临床分期显著相关,提示miR-1在食管鳞状细胞癌的发生发展中发挥着关键的作用,为进一步研究食管鳞状细胞癌发生的分子机制提供依据。Objective:To determine the expression profiles of miR-1 in esophageal squamous cell carcinoma(ESCC)and the clinical significance of the downregulation of miR-1 in primary ESCC.Methods:The expression profiles of miR-1 in 64 pairs of ESCC tissues and 5 ESCC cell lines were analyzed by reverse transcription PCR and SYBR green quantitative PCR.The differences in the expression level of miR-1 and the clinicopathological characteristics were statistically analyzed by SPSS,t-test and ANOVA test.Results:Among all the samples,90.6%(58/64)of the ESCC tissue samples showed a 2-fold lower expression of miR-1 than that in normal tissues.And miR-1 was significantly downregulated in 5 ESCC cell lines.The results revealed that the downregulation of miR-1 significantly correlated with tumor invasion and advanced clinical stage,while no significant correlation with gender,age,tumor size and degree of differentiation.Conclusion:The expression of miR-1 in ESCC is downregulated and significantly correlated with the depth of tumor invasion and clinical stage.These findings suggest that miR-1 play important roles in the development and progression of ESCC which provides a basic for further reseach on the molecular mechanism of ESCC.

关 键 词：食管鳞状细胞癌 MIR-1 实时定量荧光PCR 

分 类 号：R735.1[医药卫生—肿瘤]