16S rRNA测序分析重症胰腺炎患者外周血细菌谱  

Characterization of blood bacterial profiles in patients with severe acute pancreatitis using the 16S rRNA gene next-generation sequencing

作　　者：王洁[1] 王玉[1] 张家勋 曹进 陈勇 孙宁 Wang Jie;Wang Yu;Zhang Jiaxun;Cao Jin;Chen Yong;Sun Ning(Clinical Medicine Research Central,the Affiliated Suqian First People′s Hospital of Nanjing Medical University,Suqian 223800,China;Department of Clinical Laboratory,General Hospital of Eastern Theater Command,Nanjing 210002,China)

机构地区：[1]南京医科大学附属宿迁第一人民医院临床医学研究中心,宿迁223800 [2]东部战区总医院检验科,南京210002

出　　处：《实用医技杂志》2024年第11期775-778,I0001,共5页Journal of Practical Medical Techniques

基　　金：江苏省宿迁市科技计划项目(SY202214);江苏省卫生健康委员会医学科研项目(M2021098)。

摘　　要：目的研究重症胰腺炎(SAP)患者外周血细菌谱,探讨细菌易位与胰腺感染之间的相关性。方法收集东部战区总医院2020—2021年45例SAP患者外周血标本,外周血标本和胰液常规检测标本采集时间为同一天。提取标本DNA,采用聚合酶链反应(PCR)扩增,进行16S rRNA高通量测序(NGS)。结果来自15例SAP患者的17例次外周血标本成功进行了16S rRNA NGS检测,并检测到了57株细菌,分属于25个属27个种。奥巴涅分枝杆菌和约氏不动杆菌检出率分别为52.9%(9/17)和35.3%(6/17)。与相应胰液标本的检测结果相比,两者具有极大的差异性,仅在2对标本(胰液和外周血)中检测2个相同的细菌。结论SAP患者外周血与胰腺感染的细菌种类可能存在极大的差异,血液循环可能不是细菌易位的主要途径。Objective To characterize the bacterial profile presenting in peripheral blood of patients with severe acute pancreatitis(SAP)and the correlation between bacterial translocation and pancreatic infection.Methods The peripheral blood samples were collected from 45 patients whose pancreatic fluid samples were collected for routine microbiological test from 2020 to 2021 in General Hospital of Eastern Theater Command.Genomic DNA was extracted from peripheral blood sample,and detected using the 16S rRNA gene next-generation sequencing(NGS).Results Seventeen peripheral blood samples collected from 15 severe pancreatitis patients were successfully determined using 16S rRNA gene NGS.Fifty-seven bacterial strains were detected which belongs to 27 species in 25 genera.The detection rates of Mycobacterium aubagnense and Acinetobacter johnsonii were 52.9%(9/17)and 35.3%(6/17),respectively.Compared with the results of corresponding pancreatic fluid samples,the bacterial profile of peripheral blood was significant different.Only two bacterial strains were detected in both peripheral blood and pancreatic fluid.Conclusion There is significant difference on bacterial profile between in the peripheral blood and in the pancreatic fluid samples for SAP patients.Hematogenous dissemination may not be the main way for bacterial translocation.

关 键 词：高通量测序 重症胰腺炎 细菌谱 细菌易位 

分 类 号：R576[医药卫生—消化系统]