Development of ratiometric DNA biosensors with improved accuracy,precision,and signal-to-noise ratio  

作　　者：Qing Tao Yanli Wen Wen Liang Lele Wang Ruiyan Guo Min Ding Ming Luo Juan Yan Feiyan Gong Chengming Cao Lanying Li Gang Liu 

机构地区：[1]Key Laboratory of Bioanalysis and Metrology for State Market Regulation,Shanghai Institute of Measurement and Testing Technology,Shanghai 201203,China [2]Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation(Shanghai),Ministry of Agriculture,Shanghai Engineering Research Center of Aquatic-Product Process&Preservation,College of Food Science and Technology,Shanghai Ocean University,Shanghai 201306,China

出　　处：《Advanced Sensor and Energy Materials》2024年第3期11-22,共12页先进传感器与能源材料（英文）

基　　金：financially supported by:The National Quality Infrastructure Program of China(2021YFF0600705 NQI);the Natural Science Foundation of China(No.22074093).

摘　　要：Ratiometric DNA biosensors,which utilize DNA as the recognition element and integrate dual signals from diverse sensing platforms including fluorescence,electrochemistry,and surface-enhanced Raman scattering(SERS),have demonstrated remarkable proficiency in detecting a wide range of targets.In this review,we showcase the significant progress achieved by ratiometric biosensors.Firstly,ratiometric biosensors have made notable advancements in analyzing real samples.These include the analysis of pH values near cancer cells,quantification of miRNA in human cell lysates,detection of human telomerase RNA in cell extracts,and performing DNA logicgated in situ bioimaging on cell membranes.Secondly,excellent sensitivity has been attained through the utilization of effective amplification methods such as RCA,HCR,and CHA,among others.Thirdly,the construction of stable reference signals has resulted in significantly improved precision for ratiometric biosensors.This breakthrough has overcome matrix effects,enabling reliable detection in real samples with high selectivity.This review provides a comprehensive overview of recent advancements in strategies employed by ratiometric DNA biosensors.We present three types of biosensors based on distinct sensing platforms:fluorescent,electrochemical,and SERS biosensors.Additionally,we discuss future directions and primary challenges in the development of ratiometric DNA biosensors.

关 键 词：Ratiometric biosensor DNA NANOMATERIALS Fluorescent biosensor Electrochemical biosensor SERS biosensor 

分 类 号：R73[医药卫生—肿瘤]