白藜芦醇对鼠伤寒沙门氏菌诱导型IPEC-J2细胞损伤的缓解作用  

Alleviating effect of resveratrol on Salmonella typhimurium-induced damage in IPEC-J2 cell

作　　者：赵峥 余秋颖 杨聪燕[3,4,5] 李珊珊 陈琳琳 王娜[2,3,4,5] ZHAO Zheng;YU Qiuying;YANG Congyan;LI Shanshan;CHEN Linlin;WANG Na(College of Food Science and Technology,Henan Agricultural University,Zhengzhou 450002,China;Zhengzhou Nutrition and Health Food Laboratory,Zhengzhou 450002,China;Longhu Laboratory,Zhengzhou 450046,China;International Joint Research Center of Animal Immunology,Henan Agricultural University,Zhengzhou 450046,China;College of Animal Medicine,Henan Agricultural University,Zhengzhou 450046,China)

机构地区：[1]河南农业大学食品科学技术学院,河南郑州450002 [2]郑州市营养与健康食品重点实验室,河南郑州450002 [3]龙湖现代免疫实验室,河南郑州450046 [4]河南农业大学动物免疫学国际联合研究中心,河南郑州450046 [5]河南农业大学动物医学院,河南郑州450046

出　　处：《河南农业大学学报》2024年第6期957-965,共9页Journal of Henan Agricultural University

基　　金：河南省重大科技专项(221100110600);河南省创新生态支撑专项(HARS-22-05-Z1)。

摘　　要：【目的】研究白藜芦醇(resveratrol, RES)对鼠伤寒沙门氏菌(Salmonella typhimurium)诱导猪小肠上皮细胞(intestinal porcine epithelial cells, IPEC-J2)损伤的缓解作用,为促进动物健康养殖提供新思路。【方法】用鼠伤寒沙门氏菌建立IPEC-J2损伤模型,测定细胞上清液一氧化氮(NO)浓度、肿瘤坏死因子-α(TNF-α)质量浓度、白介素-6(IL-6)质量浓度、白介素-4(IL-4)质量浓度、乳酸脱氢酶(lactate dehydrogenase, LDH)水平、总超氧化物歧化酶(total superoxide dismutase, T-SOD)活力、还原型谷胱甘肽(glutathione, GSH)质量浓度和丙二醛(malondialdehyde, MDA)浓度,分析RES对IPEC-J2损伤模型的缓解作用。【结果】RES预处理IPEC-J2细胞的最佳质量浓度为7.8 mg·L^(-1),且能有效缓解鼠伤寒沙门氏菌诱导IPEC-J2细胞产生的炎症损伤,显著降低促炎因子(TNF-α和IL-6)的质量浓度,提高抑炎因子(IL-4)的表达,进而有效减缓肠道炎症反应。同时,RES可显著提高T-SOD活力和GSH质量浓度,显著降低MDA浓度,缓解鼠伤寒沙门氏菌诱发的氧化损伤,达到维持机体肠道氧化还原平衡的稳态。【结论】RES对IPEC-J2损伤具有保护作用,可用于预防鼠伤寒沙门氏菌感染。【Objective】To investigate the protective effects of resveratrol(RES)against oxidative and inflammatory damage induced by Salmonella typhimurium in intestinal porcine epithelial(IPEC-J2)cells and provide insights to enhance animal health in breeding practices.【Method】An IPEC-J2 cell injury model was established using S.typhimurium,and key oxidative stress and inflammatory indicators were measured in the cell supernatant,including nitric oxide(NO)content,tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),interleukin-4(IL-4)secretion,lactate dehydrogenase(LDH)level,total superoxide dismutase(T-SOD),reduced glutathione(GSH)and malondialdehyde(MDA),to analyze the mitigating effects of RES on IPEC-J2 cell injury model.【Result】Pretreatment of IPEC-J2 cells with RES at an optimal mass concentration of 7.8 mg·L^(-1) could significantly alleviate the inflammatory injury induced by S.typhimurium,notably reducing the contents of pro-inflammatory factors(TNF-αand IL-6),and increasing the expression of inhibitory factors(IL-4),thus effectively slowing down the intestinal inflammatory response.Meanwhile,RES could significantly increase the activities of T-SOD and GSH enzymes,significantly reduce the expression level of MDA,and alleviate the oxidative damage induced by S.typhimurium,thereby contributing to the maintenance of intestinal redox homeostasis of the organism.【Conclusion】RES has a protective effect on IPEC-J2 cell damage and holds potential for preventing S.typhimurium infection in animal models.

关 键 词：白藜芦醇 鼠伤寒沙门氏菌 IPEC-J2细胞 炎症反应 氧化应激 

分 类 号：TS209[轻工技术与工程—食品科学]