农药降解菌的筛选及应用评价  

Screening and application evaluation of pesticide degrading strain

作　　者：孔洁 樊梅娜 陶宁 韩广泉 冉新炎 王丽荣 KONG Jie;FAN Meina;TAO Ning;HAN Guangquan;RAN Xinyan;WANG Lirong(Shandong Bee-lan Biotechnology Co.,Ltd.,Shandong Provincial Agricultural Microbial Technology Innovation Center,Key Laboratory of Agricultural Microbial Resources and Utilization in Huanghuaihai,Shandong Tai'an 271000,China)

机构地区：[1]山东碧蓝生物科技有限公司/山东省农业微生物技术创新中心/黄淮海农业微生物资源与利用重点实验室,山东泰安271000

出　　处：《现代农药》2024年第6期58-62,72,共6页MODERN AGROCHEMICALS

基　　金：山东省科技型中小企业创新能力提升工程项目(2022TSGC1301);2020年度泰山英才领军人才项目(室字[2021]5号)。

摘　　要：采用富集驯化、分离纯化法从受农药污染的土样和水样中筛选分离得到1株农药降解菌NBL-B0078,并通过形态学和生理生化特征,以及16S rRNA基因序列分析进行鉴定,同时,考察了其对作物的安全性,及对土壤和萝卜中农药残留的降解能力。结果表明,菌株NBL-B0078对百菌清、敌敌畏、高效氯氰菊酯、2,4-滴、烯酰吗啉具有良好的降解能力,降解率分别达到100%、63.64%、75.00%、69.23%和83.54%;经分子鉴定,NBL-B0078为枯草芽孢杆菌斯氏亚种(Bacillus subtilis subsp. spizizenii)。该降解菌株能够显著降低土壤和萝卜中农药残留量,是一株具有良好开发前景的农药降解菌。A pesticide degrading strain NBL-B0078 was isolated from soil and water contaminated with pesticides by enrichment domestication,isolation and purification.It was identified by morphological,physio-biochemical,16S rRNA sequence analysis.And the safety of NBL-B0078 to crops and its abilities to degrade pesticide residues in soil and radish were investigated.The rusults showed that strain NBL-B0078 had good degradation ability,the degradation rates to chlorothalonil,dichlorvos,beta-cypermethrin,2,4-D and dimethomorph were 100%,63.64%,75.00%,69.23%and 83.54%,respectively.After molecular identification,strain NBL-B0078 was identified as Bacillus subtilis subsp.spizizenii.The degrading strain could significantly reduce pesticide residues in soil and radish,and it had good development prospect.

关 键 词：农药降解菌 枯草芽孢杆菌斯氏亚种 农药残留 百菌清 筛选 

分 类 号：X172[环境科学与工程—环境科学] X592