黄芩对斑点叉尾鮰生长性能、血清和肝脏生化指标及肝脏组织学的影响  

Effects of dietary Chinese herbal medicine Scutellaria baicalensis Georgi on growth performance,serum and liver biochemical indices and liver histology of channel catfish(Ictalurus punctatus)

作　　者：刘浩楠 冷向军[1,2,3] 游宇海 刘佳玮 陈云峰 李小勤 LIU Haonan;LENG Xiangjun;YOU Yuhai;LIU Jiawei;CHEN Yunfeng;LI Xiaoqin(National Demonstration Center for Experimental Fisheries Science Education,College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201306,China;Centre for Research on Environmental Ecology and Fish Nutrition(CREEFN),Ministry of Agriculture and Rural Affairs,Shanghai 201306,China;Shanghai Collaborative Innovation Center for Aquatic Animal Genetics and Breeding,Shanghai 201306,China)

机构地区：[1]上海海洋大学水产与生命学院,水产科学国家级实验教学示范中心,上海201306 [2]农业农村部鱼类营养与环境生态研究中心,上海201306 [3]水产动物遗传育种中心上海市协同创新中心,上海201306

出　　处：《大连海洋大学学报》2024年第5期739-745,共7页Journal of Dalian Ocean University

基　　金：国家重点研发计划“蓝色粮仓科技创新”重点专项(2019YFD0900200)。

摘　　要：为研究中草药黄芩(Scutellaria baicalensis Georgi)对斑点叉尾鮰(Ictalurus punctatus)生长性能、抗氧化和免疫性能力的影响,在基础饲料中分别添加0(对照组)、5、10、15 g/kg黄芩,配制成4组等氮等脂饲料,选取360尾平均体质量为(4.68±0.02)g的斑点叉尾鮰,分为4组,每组设置3个重复,每个重复30尾鱼,在网箱中养殖60 d;试验结束后,测定不同黄芩含量添加组斑点叉尾鮰的生长性能、血清非特异性免疫能力和肝脏抗氧化能力,并观察肝脏组织学的变化。结果表明:与对照组相比,饲料中黄芩添加量分别为5、10 g/kg时,斑点叉尾鮰的终末体质量(FBW)、增重率(WGR)和特定生长率(SGR)在数值上有所提高,但无显著性差异(P>0.05);黄芩添加量为10 g/kg时,饲料系数(FCR)显著降低(P<0.05);饲料中黄芩添加量为5、10 g/kg时,可显著提升斑点叉尾鮰血清LZM、ACP和TP水平,以及肝脏T-AOC、CAT和SOD水平(P<0.05),显著降低血清ALT、AST、TCHO水平和肝脏MDA水平(P<0.05);添加不同水平的黄芩均降低了肝脏的空泡化率。研究表明,在饲料中添加黄芩可显著提高斑点叉尾鮰对饲料的利用效率,同时增强机体的抗氧化能力,本试验条件下饲料中适宜的黄芩添加量为10 g/kg。In order to investigate the effects of dietary Chinese herbal medicine(Scutellaria baicalensis Georgi)on growth performance,antioxidant and non-specific immunity capacity of channel catfish Ictalurus punctatus,juvenile channel catfish with body weight of(4.68±0.02)g were reared in net cages of each 1.0 m×1.0 m×1.2 m and fed isonitrogen and isolipid basic diet containing 0(control group),5,10 and 15 g/kg S.baicalensis for 60 days with 3 replicates per group and 30 individuals per replicate.Then the growth performance,serum non-specific immune capacity and antioxidant capacity in liver were determined,and the histological structure was observed in the liver of the channel catfish.The results showed that there were increase in final body weight,weight gain rate and specific growth rate in 5 g/kg and 10 g/kg Chinese herbal medicine groups,without significant difference(P>0.05)compared with those in the control group.The food conversion ratio,however,was found to be significantly decreased by the supplementation of 10 g/kg Chinese herbal medicine(P<0.05).The supplementation of 5 g/kg and 10 g/kg Chinese herbal medicine led to significantly increase the levels of lysozyme acid phosphatase,total protein in serum and the levels of total antioxidant capacity,catalase,and superoxide dismutase in liver(P<0.05),and to decrease the levels of alanine aminotransferase,aspartate aminotransferase,and total cholesterol content in serum and malondialdehyde content in liver(P<0.05),with low vacuolation rate of liver.The findings indicate that the supplementation of S.baicalensis in diets can improve the feed utilization efficiency and enhance the antioxidant activity of channel catfish,with the appropriate supplemental amount of S.baicalensis of 10 g/kg under the experimental conditions.

关 键 词：斑点叉尾鮰 黄芩 生长性能 血清生化指标 抗氧化水平 

分 类 号：S965.128[农业科学—水产养殖]