Investigating efficacy mechanism of electroacupuncture in treating Parkinson disease through TMT proteomics  

基于TMT蛋白质组学探讨电针治疗帕金森病的疗效机制

作　　者：ZHU Lu LI Guona WU Pin WU Luyi SHEN Lin QIAO Yu LI Jing LI Lingjie WANG Zhaoqin CHEN Yiyi MA Xiaopeng LI Kunshan WU Huangan 朱璐;李国娜;吴玭;吴璐一;沈琳;乔宇;李璟;李灵杰;王照钦;陈奕奕;马晓芃;李昆珊;吴焕淦(Shanghai Research Institute of Acupuncture and Meridian,Shanghai 200030,China;Yueyang Hospital of Integrated Traditional Chinese and Western Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 200437,China;Community Health Service Center of Malu Town,Jiading District,Shanghai,Shanghai 201801,China;Shanghai Municipal Hospital of Traditional Chinese Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 200071,China)

机构地区：[1]Shanghai Research Institute of Acupuncture and Meridian,Shanghai 200030,China [2]Yueyang Hospital of Integrated Traditional Chinese and Western Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 200437,China [3]Community Health Service Center of Malu Town,Jiading District,Shanghai,Shanghai 201801,China [4]Shanghai Municipal Hospital of Traditional Chinese Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 200071,China

出　　处：《Journal of Acupuncture and Tuina Science》2024年第6期470-481,共12页针灸推拿医学（英文版）

基　　金：上海市老龄化和妇儿健康研究专项,No.2020YJZX0134;国家自然科学基金青年科学基金项目,No.82205260;上海市科学技术委员会科研计划项目,No.18401970700;上海市卫生健康委员会科研课题,No.20204Y0271;国家中医药管理局高水平中医药重点学科(针灸学)建设项目,No.ZYYZDXK-2023068.

摘　　要：Objective:To explore the therapeutic mechanism of electroacupuncture(EA)in treating Parkinson disease(PD)using Tandem mass tag(TMT)quantitative proteomics technology.Methods:Forty-eight PD patients were randomly divided into a control group and an observation group,with 24 patients in each group.The control group received routine drug treatment,while the observation group received EA in addition to the routine drug treatment.EA was administered for 30 min per session,3 times a week,for a total of 12 weeks.Nine patients from each group were randomly selected to provide peripheral blood serum samples before and after treatment for TMT quantitative proteomics analysis.Differentially expressed proteins between the two groups were compared,and bioinformatics analysis was performed.The screened differentially expressed proteins were validated using enzyme-linked immunosorbent assay(ELISA).Results:In the observation group,scores on the unified Parkinson disease rating scale(UPDRS),UPDRSⅡ,and UPDRSⅢwere significantly reduced after treatment(P<0.05).In the control group,these scores tended to increase,but the changes were not statistically significant(P>0.05).After treatment,the UPDRS and UPDRSⅢscores in the observation group were significantly lower than those in the control group(P<0.05).The observation group showed 62 differentially expressed proteins,while the control group had 36.Compared to the control group,the observation group had 142 differentially expressed proteins.These proteins were primarily involved in the cyclic adenosine monophosphate(cAMP)signaling pathway,T helper(Th)1 and Th2 cell differentiation,ATP-binding cassette transporter,vascular endothelial growth factor signaling pathway,and high-affinity immunoglobulin E receptor(FcεRI)signaling pathway.ELISA verification indicated that after EA treatment,the levels ofα-Synuclein(αSyn)and heat shock protein beta 1(HSPB1)in the observation group were significantly lower than those in the control group(P<0.05),while the regulator of G-protein signali目的:应用串联质谱标签(TMT)定量蛋白质组学技术探讨电针治疗帕金森病(PD)的疗效机制。方法:将48名PD患者根据随机数字表法分为对照组和观察组,每组24例。对照组接受常规药物治疗,观察组在常规药物治疗基础上加用电针治疗。电针每次治疗30 min,每周治疗3次,共治疗12周。每组随机选取9名患者于治疗前后采集外周血血清样本进行TMT定量蛋白质组学分析,对比两组差异蛋白质的表达情况,并进行生物信息学分析。对筛选的差异蛋白进行酶联免疫吸附试验(ELISA)验证。结果:与本组治疗前相比,观察组统一帕金森病评分量表(UPDRS)、UPDRS第Ⅱ部分(UPDRSⅡ)和UPDRS第Ⅲ部分(UPDRSⅢ)评分显著降低(P<0.05);对照组UPDRS、UPDRSⅡ和UPDRSⅢ评分有升高的趋势,但差异无统计学意义(P>0.05)。治疗后观察组UPDRS和UPDRSⅢ评分显著低于对照组(P<0.05)。与治疗前相比,观察组有62个差异蛋白,对照组有36个差异蛋白;治疗后,与对照组相比,观察组有142个差异蛋白。差异蛋白主要涉及环磷酸腺苷(cAMP)信号通路、辅助性T细胞(Th)1和Th2细胞分化、ATP-结合盒转运蛋白、血管内皮生长因子信号通路和免疫球蛋白E高亲和力受体(FcεRI)信号通路等。ELISA验证发现,经电针治疗后观察组α-突触核蛋白(αSyn)、热休克蛋白B1(HSPB1)含量显著低于对照组(P<0.05),G蛋白信号调节因子10(RGS10)含量显著高于对照组(P<0.05)。结论:常规药物治疗基础上加用电针治疗可以更好地改善PD患者临床症状;其作用机制可能与调节cAMP信号通路和差异蛋白αSyn、HSPB1及RGS10含量有关。

关 键 词：Acupuncture Therapy ELECTROACUPUNCTURE PROTEOMICS Tandem Mass Spectrometry Cyclic AMP Parkinson Disease 

分 类 号：R246.6[医药卫生—针灸推拿学]