To Explore the Test Method of Eye Stimulation of Cosmetics by Zebrafish Embryo Method  

作　　者：Lei Cuiting Chen Laicheng Yang Rongxin Shen Guofeng Zheng Muchuang Xu Qitian Ma Manhua Chen Libing 

机构地区：[1]Guangdong North Indust Trial and Commercial Institutes of Green Skincare Co.,Ltd.,China [2]Guangdong Yalijie Fine Chemical Co.,Ltd.,China

出　　处：《China Detergent & Cosmetics》2024年第4期47-51,共5页日用化学品科学（英文版）

摘　　要：To study the feasibility of zebrafish model as an evaluation method for eye irritation of cosmetics,and to evaluate eye irritation of several common surfactants and wash products by this method.To construct a zebrafish eye stimulation model,stereomicroscope was used to observe the changes in eye morphology of zebrafish embryos to evaluate the stimulation of subjects.Two evaluation indexes were selected:pupil diameter and the ratio of pupil diameter to iris bandwidth.The results show that the ratio method of pupil diameter to iris bandwidth of zebrafish is insufficient.The experimental results of pupil diameter of zebrafish were highly coincident with those of chick embryo chorio-allantoic membrane.At the same time,the subject can inhibit the pupil diameter of zebrafish,and the pupil diameter is negatively correlated with the irritation of the subject.Therefore,the zebrafish model can be used in the evaluation of cosmetic eye irritation to provide rapid and efficient detection for product development.

关 键 词：eye irritation ZEBRAFISH pupil diameter 

分 类 号：TQ658[化学工程—精细化工]