Study on the Technical Parameters for Estimating HIV-1 Incidence by Using a Recombinant Antigen-based Capture Enzyme Immunoassay—China  

作　　者：Wenli Liang Jibao Wang Hongxia Yan Xinhui Zhang Dorjiwangmo Dongmin Li Xing Duan Hao Wu Yinyin Wang Li Bai Jian Sun Mengjie Han Yikui Wang Bin Su Min Wang Tashibazong Wenge Xing Cui Zhang Ruijuan Qiao Maofeng Qiu 

机构地区：[1]National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases,National Center for AIDS/STD Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing,China [2]Department of AIDS/STD Control and Prevention,Dehong Center for Disease Control and Prevention,Mangshi County,Dehong Dai and Jingpo Autonomous Prefecture,Yunnan Province,China [3]Beijing Key Laboratory for HIV/AIDS Research,Beijing Youan Hospital,Capital Medical University,Beijing,China [4]Institute of Infectious Diseases Prevention and Treatment,Guizhou Provincial Center for Disease Control and Prevention,Guiyang City,Guizhou Province,China [5]Department of AIDS Control and Prevention,Xizang Autonomous Region Center for Disease Control and Prevention,Lhasa City,Xizang Autonomous Region,China [6]Experimental Center,Guizhou Provincial Center for Disease Control and Prevention,Guiyang City,Guizhou Province,China [7]Department of AIDS Control and Prevention,Gansu Provincial Center for Disease Control and Prevention,Lanzhou City,Gansu Province,China [8]Laboratory Department,Liupanshui Center for Disease Control and Prevention,Liupanshui City,Guizhou Province,China

出　　处：《China CDC weekly》2024年第48期1278-1282,共5页中国疾病预防控制中心周报（英文）

基　　金：Supported by the National Science and Technology Major Project of the 13th Five-year Plan(grants 2017ZX10201101-002-003 and 2018ZX10721102-003-002)。

摘　　要：Introduction:A novel recombinant antigen-based capture enzyme immunoassay(RAg-CEIA)was optimized and used to determine technical parameters for estimating human immunodeficiency virus type 1(HIV-1)incidence in China.Methods:We employed orthogonal experimental design to optimize RAg-CEIA by adjusting raw material dilution ratios.The assay was used to measure normalized optical density(ODn)values in 171 longitudinal plasma specimens from 51 HIV-1 seroconverting individuals,plotted against estimated days post-seroconversion.We determined the optimal ODn threshold value for differentiating recent from long-term infections and calculated the mean duration of recent infection(MDRI)for incidence estimation.The false recent rate(FRR)was determined using 481 HIV-1 antibody-positive specimens with infection durations exceeding twice the MDRI.Results:Optimal RAg-CEIA parameters were established with a raw material dilution ratio of 1/12 for calibrator preparation and an enzyme conjugate titer of 1:1200.ODn values demonstrated consistent temporal increases across HIV-1 seroconverting individuals,though with notable kinetic heterogeneity in individual responses.The optimal ODn threshold value of 0.8 for distinguishing recent from long-term infections corresponded to an MDRI of 205 days and an FRR of 4.78%.Conclusions:The optimized RAg-CEIA effectively differentiates recent from long-term HIV-1 infections at the population level,enabling reliable HIV-1 incidence estimation in China.

关 键 词：ANTIGEN EXCEEDING false 

分 类 号：R512.91[医药卫生—内科学]