沉默CD147基因对姜黄素抑制前列腺癌细胞增殖、迁移、侵袭和诱导凋亡的影响  

Effect of silencing CD147 gene on proliferation,migration,invasion,and inducing apoptosis of prostate cancer cells inhibited by curcumin

作　　者：王馨 赵杰瑞 郭玉苗 陈姝彤 侯宗昊 张若文[1] WANG Xin;ZHAO Jierui;GUO Yumiao;CHEN Shutong;HOU Zonghao;ZHANG Ruowen(Department of Pathogenic Biology,School of Basic Medical Sciences,Beihua University,Jilin 132000,China;Department of Orthopedics,Affiliated Hospital,Traditional Chinese Medicine,Macao University of Science and Technology,Zhuhai 519000,China;Department of Biochemistry,School of Medical Sciences,Yanbian University,Yanji 133000,China)

机构地区：[1]北华大学基础医学院病原生物学教研室,吉林吉林132000 [2]澳门科技大学中医药学院附属医院骨外科,广东珠海519003 [3]延边大学医学院生物化学教研室,吉林延吉133000

出　　处：《吉林大学学报（医学版）》2024年第6期1572-1586,共15页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技厅科技发展计划项目(YDZJ202101ZYTS123);北华大学研究生创新项目(研创合字[2022]012)。

摘　　要：目的:探讨姜黄素对人前列腺癌C4-2细胞和LNCaP细胞增殖、迁移及侵袭的影响,并阐明其可能的作用机制。方法:采用慢病毒转染系统分别转染C4-2细胞和LNCaP细胞,作为shCD147-C4-2组和shCD147-LNCaP组。采用RNA干扰技术制备沉默CD147基因细胞,以转入空载体的细胞作为阴性对照,分为shNC-C4-2组(shNC-C4-2细胞)和shNC-LNCaP组(shNC-LNCaP细胞)。取生长对数期C4-2、LNCap、shCD147-C4-2和shCD147-LNCaP细胞,加入20μmol·L^(-1)姜黄素,处理0和24 h时,显微镜观察各组细胞形态表现。噻唑蓝(MTT)法检测各组细胞增殖活性,细胞划痕实验检测各组细胞迁移率,Western blotting法检测各组细胞中凋亡、侵袭和迁移相关蛋白表达水平。结果:与C4-2组比较,沉默CD147基因后shCD147-C4-2组细胞中CD147蛋白表达量明显减少;与LNCaP组比较,沉默CD147基因后shCD147-LNCaP组细胞中CD147蛋白表达量明显减少。与处理0 h比较,20μmol·L^(-1)姜黄素处理24 h后C4-2组和LNCaP组部分细胞出现凋亡征象,且有典型凋亡小体存在;shCD147-C4-2组和shCD147-LNCaP组细胞凋亡现象减弱。MTT法检测,与C4-2+0μmol·L^(-1)姜黄素组比较,C4-2+20μmol·L^(-1)姜黄素组、C4-2+40μmol·L^(-1)姜黄素组、C4-2+60μmol·L^(-1)姜黄素组和C4-2+80μmol·L^(-1)姜黄素组细胞增殖活性均明显降低(P<0.01);与LNCaP+0μmol·L^(-1)姜黄素组比较,LNCaP+20μmol·L^(-1)姜黄素组、LNCaP+40μmol·L^(-1)姜黄素组、LNCaP+60μmol·L^(-1)姜黄素组和LNCaP+80μmol·L^(-1)姜黄素组细胞增殖活性均明显降低(P<0.01);与shNC-C4-2组比较,shNC-C4-2+20μmol·L^(-1)姜黄素组细胞增殖活性明显降低(P<0.01);与shNC-C4-2+20μmol·L^(-1)姜黄素组比较,shCD147-C4-2+20μmol·L^(-1)姜黄素组细胞增殖活性明显升高(P<0.01);与shNC-LNCaP组比较,shNC-LNCaP+20μmol·L^(-1)姜黄素组细胞增殖活性明显降低(P<0.01);与shNC-LNCaP+20μmol·L^(-1)姜黄素组比较,shCD147-LNCaP+20μmol·L^(-1)姜黄素组细胞增殖活性�Objective:To discuss the effect of curcumin on the proliferation,migration,and invasion of the human prostate cancer C4-2 and LNCaP cells,and to clarify its possible mechanism.Methods:The lentiviral transfection system was used to transfect the C4-2 and LNCaP cells,regarded as shCD147-C4-2 group and shCD147-LNCaP group.RNA interference technology was used to prepare the CD147-silenced cells;the cells transfected with an empty vector were regarded as negative control and divided into shNC-C4-2 group(shNC-C4-2 cells)and shNC-LNCaP group(shNC-LNCaP cells).The C4-2 and LNCaP cells at logarithmic growth phase,as well as shCD147-C4-2 and shCD147-LNCaP cells,were treated with 20μmol·L^(-1)curcumin.The morphology of the cells in various groups was observed under microscope at 0 and 24 h of treatment;MTT method was used to detect the proliferation activities of the cells in various groups;cell scratch assay was used to detect the migration rates of the cells in various groups;Western blotting method was used to detect the expression levels of apoptosis,invasion,and migration-related proteins in the cells in various groups.Results:Compared with C4-2 group,the expression of CD147 protein in the cells in shCD147-C4-2 group was significantly decreased after CD147 gene silenting.Compared with LNCaP group,the expression level of CD147 protein in the cells in shCD147-LNCaP group was significantly decreased after CD147 gene silenting.Compared with 0 h of treatment,some cells in C4-2 and LNCaP groups after 24 h of treatment with 20μmol·L^(-1)curcumin,showed apoptosis signs with the presence of typical apoptotic bodies.The apoptotic phenomena in shCD147-C4-2 and shCD147-LNCaP groups was reduced.The MTT assay results showed that compared with C4-2+0μmol·L^(-1)curcumin group,the proliferation activities of the cells in C4-2+20μmol·L^(-1)curcumin group,C4-2+40μmol·L^(-1)curcumin group,C4-2+60μmol·L^(-1)curcumin group,and C4-2+80μmol·L^(-1)curcumin group were decreased(P<0.01).Compared with LNCaP+0μmol·L^(-1)curcumin gr

关 键 词：姜黄素 CD147 前列腺肿瘤 细胞侵袭 细胞迁移 

分 类 号：R735.25[医药卫生—肿瘤]