宫颈病变和宫颈癌组织中嗜酸性粒细胞浸润及其临床意义  

Eosinophil infiltration in cervical lesion and cervical cancer tissues and their clinical significances

作　　者：鲁艳艳 许翔博 吴亚梅 刘雨齐 王涵 杨丽娟[1] 王振江[1] 肖梓屾 刘艳波[1] LU Yanyan;XU Xiangbo;WU Yamei;LIU Yuqi;WANG Han;YANG Lijuan;WANG Zhenjiang;XIAO Zishen;LIU Yanbo(Department of Pathophysiology,School of Basic Medical Sciences,Beihua University,Jilin 132013,China;Department of Pathology,Gynaecology and Obstetrics Hospital,Changchun City,Jilin Province,Changchun 130028,China;Department of Gynaecology and Obstetrics,People’s Hospital,Jilin City,Jilin Province,Jilin 132011,China)

机构地区：[1]北华大学基础医学院病理生理学教研室,吉林吉林132013 [2]吉林省长春市妇产医院病理科,吉林长春130028 [3]吉林省吉林市人民医院妇产科,吉林吉林132011

出　　处：《吉林大学学报（医学版）》2024年第6期1691-1702,共12页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技厅国际合作项目(20210402015GH);吉林省科技厅基础研究项目(YDZJ202201ZYTS194);北华大学研究生创新项目(研创合字[2022]056)。

摘　　要：目的:探讨嗜酸性粒细胞(EOS)在宫颈组织中的浸润差异及其与宫颈相关疾病之间的关系,阐明EOS对宫颈上皮不典型增生(CIN)和宫颈癌发生发展的影响。方法:收集256例宫颈疾病患者的临床资料,根据其发病情况分为宫颈癌组(n=46,其中宫颈鳞状细胞癌26例、宫颈腺癌15例和宫颈腺鳞癌5例)、慢性宫颈炎组(n=50)、CINⅠ期组(n=50)、CINⅡ期组(n=50)、CINⅢ期组(n=30)和正常组(癌旁正常宫颈组织,n=30)。阴道镜观察各组患者宫颈组织形态表现,薄层液基细胞学测试(TCT)法观察各组患者宫颈脱落细胞形态表现,杂交捕获-化学发光法检测各组患者宫颈组织人乳头瘤病毒(HPV)感染情况,HE染色观察各组患者宫颈组织病理形态表现,刚果红染色检测各组患者宫颈组织中EOS浸润数,Pearson相关性分析EOS浸润数与宫颈癌恶性程度的相关性。结果:正常组患者宫颈表面光滑,呈粉红色,毛细血管均匀分布;慢性宫颈炎组患者宫颈表面呈红色炎性改变,部分伴有纳氏囊肿形成,可见不同程度的糜烂和溃疡等;CINⅠ期、CINⅡ期和CINⅢ期组患者宫颈可见上皮溃疡、增厚和形态不规则,细镶嵌及点状血管明显;宫颈癌组患者宫颈表面隆起,可见新生肿物及坏死性溃疡,质脆易出血。醋酸染色后,正常组患者宫颈无明显改变;慢性宫颈炎组患者宫颈呈少量白色改变,持续时间较短;CINⅠ期、CINⅡ期和CINⅢ期组患者宫颈薄醋白上皮不规则、呈地图样边界,其中CINⅠ期组患者宫颈部分组织呈醋白反应,CINⅡ期组患者宫颈出现明显醋白反应,CINⅢ期组患者宫颈醋白反应非常明显,面积较大,且持续时间较长;宫颈癌组患者宫颈醋白反应明显,白色上皮厚,持续时间久,轮廓硬直,边界清晰。正常组患者宫颈碘染色后呈棕褐色,着色均匀;慢性宫颈炎组患者宫颈炎性病变区着色差;CINⅠ期组患者宫颈上皮化生区碘着色不明显;CINⅢ期组患�Objective:To discuss the differences in eosinophil(EOS)infiltration in cervical tissue and its relationship with cervical-related diseases,and to clarify the effect of EOS on the occurrence and development of cervical intraepithelial neoplasia(CIN)and cervical cancer.Methods:The clinical data of 256 patients with cervical diseases were collected and divided into cervical cancer group(n=46,including 26 cases of squamous cell carcinoma,15 cases of adenocarcinoma,and 5 cases of adenosquamous carcinoma),chronic cervicitis group(n=50),CIN stageⅠgroup(n=50),CIN stageⅡgroup(n=50),CIN stageⅢgroup(n=30),and normal group(adjacent normal cervical tissue,n=30)based on their conditions.Colposcopy was used to observe the morphology of cervical tissue of the patients in various groups;thin-layer liquid-based cytology test(TCT)was used to observe the morphology of the cervical exfoliated cells in various groups;hybrid capture-chemiluminescence method was used to detect the human papillomavirus(HPV)infection in cervical tissue of the patients in various groups;HE staining was used to observe the pathomorphology of cervical tissue of the patients in various groups;Congo red staining was used to detect the numbers of EOS infiltration in cervical tissue of the patients in various groups;Pearson correlation analysis was used to analyze the correlation between the number of EOS infiltration and the malignancy degree of cervical cancer.Results:The cervical surface of the patients in normal group was smooth and pink,with uniformly distributed capillaries;the cervical surface of the patients in chronic cervicitis group showed red inflammatory changes,with some accompanied by Nabothian cysts and varying degrees of erosion and ulcers;the patients in CIN stageⅠ,CIN stageⅡ,and CIN stageⅢgroups showed epithelial ulcers,thickening,and irregular morphology,with mosaic and punctate vessels;the cervical surface of the patients in cervical cancer group showed raised areas with neoplasms and necrotic ulcers,and they were fragile and pro

关 键 词：嗜酸性粒细胞 宫颈肿瘤 宫颈上皮不典型增生 慢性宫颈炎 细胞浸润 

分 类 号：R737.33[医药卫生—肿瘤]