芥菜型油菜苗期Cd积累相关性状全基因组关联分析  

Genome-wide association analysis of Cd accumulation-related traits of Brassica juncea in seedling stage

作　　者：李雪姣 唐静[1] 郭宝 杨依林 王茹梦 周筱逸 杨勇[1] 罗劲松 张振华[1] LI Xuejiao;TANG Jing;GUO Bao;YANG Yilin;WANG Rumeng;ZHOU Xiaoyi;YANG Yong;LUO Jinsong;ZHANG Zhenhua(College of Resources,Hunan Agricultural University,Changsha 410128,China)

机构地区：[1]湖南农业大学资源学院,长沙410128

出　　处：《植物生理学报》2024年第11期1674-1684,共11页Plant Physiology Journal

基　　金：国家重点研究计划项目课题(2022YFD1700103);国家自然科学基金面上项目(32272811);湖南省自然科学基金重大项目(2021JC0001);国家水稻产业技术体系(CARS-01-30)。

摘　　要：芥菜型油菜(Brassica juncea)因其耐贫瘠、高生物量、高Cd富集能力而油分不含Cd的特点,被视为理想的植物修复材料,但目前控制芥菜型油菜Cd积累耐受性差异的遗传调控位点仍不清楚。本研究利用162份芥菜型油菜关联群体,采用MLM和GLM模型,在10μmol·L^(-1) Cd处理下,对地上部Cd累积量(STCd)、总Cd累积量(TCd)和Cd转运系数(TF)进行全基因组关联分析,共检测到14个显著SNP,其中3个SNP位点在STCd和TCd中重复关联。结合基因功能注释和文献报道,筛选出10个与苗期Cd积累相关的候选基因。单倍型分析表明,铜伴侣蛋白(BjuCCH)的单倍型之间存在显著差异。本研究为揭示Cd积累差异的自然等位变异提供了新的研究位点。Brassica juncea may be an ideal plant for phytoremediation because it can grow in poor soils,has high biomass,and effectively accumulates cadmium(Cd)without storing it in its oil fraction.However,the genetic regulatory loci controlling the variability in cadmium accumulation tolerance in B.juncea remain unclear.This study used 162 accessions,applying MLM and GLM models under 10μmol·L^(-1) Cd treatment,to conduct a GWAS on shoot Cd accumulation(STCd),total Cd accumulation(TCd),and Cd translocation factor(TF).Fourteen significant SNPs were identified,with three repeatedly associated with STCd and TCd.Ten candidate genes related to seedling Cd accumulation were selected through gene function annotation and literature review.Haplotype analysis showed significant differences in the copper chaperone protein(BjuCCH)haplotypes.This study provides new loci for investigating natural allelic variation in Cdaccumulation.

关 键 词：芥菜型油菜 Cd累积 全基因组关联分析 

分 类 号：S565.4[农业科学—作物学]