肌底液中酵母溶胞产物和多肽组合物的作用研究  

A Study on the Efficacy of a Combination of Yeast Lysate Products and Peptide in Serum

作　　者：钟丽玉 林玉珊 闫永涛 黄楚雯 吴绮霞 贺晓静 Zhong Liyu;Lin Yushan;Yan Yongtao;Huang Chuwen;Wu Qixia;He Xiaojing(Perfect(Guangdong)Commodiyt Co.,Ltd.,Zhongshan 528400,China;Perfect Life Sciences Research Institute Co.,Ltd.,Zhongshan 528400,China)

机构地区：[1]完美(广东)日用品有限公司,广东中山528400 [2]广东完美生命健康科技研究院有限公司,广东中山528400

出　　处：《广东化工》2024年第22期68-71,共4页Guangdong Chemical Industry

摘　　要：本研究通过复配特定含量的二裂酵母发酵产物溶胞物、棕榈酰三肽-1和棕榈酰四肽-7制备出一款具有多功能皮肤功效的肌底液。通过构建小鼠巨噬细胞炎症模型、HaCaT人永生化表皮细胞划痕实验、斑马鱼eln1、eln2基因相对表达量测试和面部抗皱功效人体评价实验,评估肌底液的皮肤功效。抗炎功效实验结果表明,0.1%~0.3%浓度的肌底液可以显著减少IL-6炎症因子的释放,表现出舒缓抗炎功效;细胞体外划痕修复实验结果表明,48 h作用时间内,0.2%浓度的肌底液可以促进人上表皮细胞迁移,显现出皮肤屏障修复功效;斑马鱼eln1、eln2基因相对表达量测试数据显示0.25%以上浓度肌底液可显著增加弹性蛋白基因相对表达量,表现出紧致功效。采用Primos CR仪器科学评估使用肌底液8周的面部抗皱功效人体评价数据,发现该肌底液可以显著改善法令纹细纹和眼下细纹。In this study,a serum with multifunctional skin efficacy was prepared by compounding specific levels of bifida ferment lysate,palmitoyl tripeptide-1and palmitoyl tetrapeptide-7.The skin efficacy of the serum was evaluated by constructing a mouse macrophage inflammation model,HaCaT human immortalized epidermal cell scratch experiment,zebrafish eln1,eln2 gene relative expression test and facial anti-wrinkle efficacy human evaluation experiment.The results of the anti-inflammatory efficacy experiment showed that 0.1 %~0.3 % concentration of the serum can significantly reduce the release of IL-6 inflammatory factors,showing soothing and anti-inflammatory efficacy;the results of the in vitro cell scratch assessments showed that,0.2 % concentration of the serum can promote the migration of the human epidermal cells within 48 h,revealing the efficacy of the skin barrier repair;zebrafish eln1,eln2 gene relative expression test data show that 0.25 % or more concentration of the serum can significantly increase the relative expression of elastin genes,showing firming effect.An 8-week human evaluation data of the anti-wrinkle efficacy of the serum was scientifically assessed using the Primos CR instrument,and it was found that the serum could significantly improve the appearance of fine lines and under-eye wrinkles.

关 键 词：二裂酵母发酵产物溶胞物 生物活性肽 化妆品功效 

分 类 号：TQ658[化学工程—精细化工]