混合益生菌发酵对芍药甘草汤有效成分的影响  

Effect of Mixed Probiotics Fermentation on Active Components ofShaoyaogancao Decoction

作　　者：侯春久[1] 苏慧 敖翔 纪德佳 Hou Chunjiu;Su Hui;Ao Xiang;Ji Dejia(Jiangxi College of Traditional Chinese Medicine College of Pharmacy,Fuzhou 344000,China)

机构地区：[1]江西中医药高等专科学校,江西抚州344000

出　　处：《广东化工》2024年第23期136-139,共4页Guangdong Chemical Industry

基　　金：江西省教育厅科学技术研究项目(GJJ2205917)

摘　　要：目的:通过实验确定混合益生菌发酵对芍药甘草汤中有效成分的影响。方法:采用混合益生菌冻干粉对芍药甘草(白芍∶炙甘草=1∶1)粉末进行发酵,以白芍、炙甘草粉末为混合益生菌的培养基,利用紫外分光光度法(UV)和高效液相色谱法(HPLC)对水煎液和发酵液进行比较,以白芍中芍药苷和甘草中甘草酸为对照品,对成分含量变化进行分析。结果:UV法和HPLC法数据均显示益生菌冻干粉对芍药甘草发酵液的发酵作用明显,其中HPLC法测得芍药苷在芍药甘草汤水煎液中含量平均值为0.892 mg·mL^(-1),在发酵液中含量平均值为73.100 mg·mL^(-1);甘草苷在芍药甘草汤水煎液中含量平均值为0.156 mg·mL^(-1),在发酵液中含量平均值为50.100 mg·mL^(-1)。结论:益生菌冻干粉能够促进白芍和炙甘草中的有效成分的溶出,说明益生菌发酵可促进中药有效成分在胃肠道中的吸收,益生菌发酵中药复方芍药甘草汤具有可行性。Objective:To determine the effect of mixed probiotics fermentation on the active components in Shaoyao Gancao decoction.Methods:Thelyophilized powder of mixed probiotics was used to treat Paeonia lactiflora and Licorice Paeony:The decoction and fermentation liquid were compared by ultravioletspectrophotometry(UV)and high performance liquid chromatography(HPLC).The content changes of paeoniflorin in paeoniae paeoniae and glycyrrhizic acid inliquorice were used as control.Results:The results of UV method and HPLC method showed that the lyophilized probiotics had a significant effect on the fermentationliquid of paeoniflorin.The average content of paeoniflorin in the decoction of Paeoniflorin and the fermentation liquid was 0.892 mg·mL^(-1)and 73.100 mg·mL^(-1),respectively,measured by HPLC method.The average content of liquiritin in Shaoyao Licorice decoction was 0.156 mg·mL^(-1),and the average content of liquiritin infermentation broth was 50.100 mg·mL^(-1).Conclusion:The lyophilized probiotic powder can promote the dissolution of the active components in Paeony and friedlicorice,indicating that the fermentation of probiotics can promote the absorption of the active components in the gastrointestinal tract,and the fermentation of thetraditional Chinese medicine compound Shaoyao-Gancao decoction by probiotics is feasible.

关 键 词：益生菌 芍药甘草 发酵 

分 类 号：TQ[化学工程]