柱前衍生高效液相色谱-串联质谱测定糕点中丁二酮  

Determination of Butanedione in Pastry by Pre-column Derivatization High Performance Liquid Chromatography-mass

作　　者：魏金斌 Wei Jinbin(Fujian Inspection and Research Institute for Product Quality,National Quality Supervision and Testing Center for Processed Food(Fuzhou),Fuzhou 350000,China)

机构地区：[1]福建省产品质量检验研究院国家加工食品质量检验检测中心(福州),福建福州350000

出　　处：《广东化工》2024年第21期143-145,共3页Guangdong Chemical Industry

摘　　要：建立了柱前衍生高效液相色谱-串联质谱测定糕点中2,3-丁二酮分析方法。在弱酸性条件下,丁二酮与2,4-二硝基苯肼发生衍生反应后,采用HSS T3色谱柱分离,在梯度洗脱条件下进行分析,外标法定量。丁二酮在0.81 ng/mL~52.0 ng/mL浓度范围内线性相关系数良好(r≥0.999),方法的定量限为4.5μg/kg,加标回收率为87.0%~93.8%,相对标准偏差为2.7%~3.3%。该方法操作简便、灵敏度高,可应用于糕点样品中丁二酮含量的测定。A method for the determination of butanedione(2,3-butanedione) in pastry by pre-column derivatization high performance liquid chromatography-mass.Butanedione from pastry can react with 2,4-dinitrophenyl hydrazine under weak acid condition.The target analyte was separated by HSS T3column with gradient elution using 0.1% formic acide solution and acetonitrile,which was quantified by external standard method.The experimental results demonstrated that the target analyte has good linearity in the concentration range of 0.81 ng/mL~52.0 ng/mL with correlation coefficients(r) above 0.999.The limit of quantitation was 4.5 μg/kg,and the recoveries were in the range 87.0 %~93.8 %,with the relative standard deviations were 2.7 %~3.3 %.The method was simplicity,high sensitivity and suitable for the quantitative of butanedione in pastry products.

关 键 词：柱前衍生 高效液相色谱-串联质谱 糕点 丁二酮 

分 类 号：TQ651[化学工程—精细化工]