复合地龙合剂对小鼠抑郁症的影响  

Effect of Dilong composite agentia on major depressive disorder in mice

作　　者：沐奕岚 韩丽[1] 蒋彭成[1] 魏斯文 罗怀青 MU Yilan;HAN Li;JIANG Pengcheng;WEI Siwen;LUO Huaiqing(The First Clinical College,Changsha Medical College,Changsha 410219,China;School of Medicine,Hunan Normal University,Changsha 410013,China;School of Medicine,Jishou University,Jishou 416000,China)

机构地区：[1]长沙医学院第一临床学院,长沙410219 [2]湖南师范大学医学院,长沙410013 [3]吉首大学医学院,吉首416000

出　　处：《湖南师范大学学报（医学版）》2024年第4期12-17,共6页Journal of Hunan Normal University(Medical Sciences)

基　　金：国家级大学生创新创业训练计划项目“复合地龙剂对小鼠抑郁症的影响及其机制研究”[教高司函(2022)10号-202210823004]。

摘　　要：目的:探索复合地龙合剂(compound Dilong agentia,DCA)对抑郁症(major depressive disorder,MDD)小鼠的影响。方法:采用慢性不可预测应激模型法(chronic unpredictable mild stress,CUMS)法构建MDD模型。小鼠分为control组、MDD组、MDD+paroxetine组、MDD+DCA组。采用强迫游泳实验、悬尾实验、糖水偏好实验评估小鼠抑郁样行为;HE染色检测小鼠海马CA1区神经元病理变化;免疫组化检查海马NF-κB的表达;WB检测海马BDNF表达;ELISA法检测TNF-α、IL-1β和IL-6水平。结果:MDD+DCA组小鼠体重显著高于MDD组。MDD+DCA组小鼠糖水偏好率显著高于MDD组。强迫游泳实验和悬尾实验结果均显示,MDD+DCA组小鼠不动时间显著短于MDD组。HE染色和免疫组化结果显示,DCA可显著减轻CUMS法诱导的MDD小鼠海马CA1神经元病理改变,降低NF-κB的表达。MDD+DCA组小鼠BDNF蛋白表达显著高于MDD组。MDD+DCA组小鼠脑组织中TNF-α、IL-1β和IL-6表达显著低于MDD组。结论:复合地龙合剂能降低MDD模型小鼠脑组织炎症反应,提高其海马组织中BDNF表达,促进神经修复,显著改善抑郁样行为。Objective To explore the effects of Dilong Composite Agentia (DCA) on mice with major depressive disorder (MDD).Methods The classical method-chronic unpredictable mild stress (CUMS) was used to construct MDD model.In the experiment,mice were evenly divided into control group,MDD group,MDD+ Paroxetine group and MDD + DCA group.We used the following techniques to indicate the results:sugar water preference experiments,tail suspension experiments,and forced swimming experiments for behavioral testing;HE staining to examine the pathological alterations in the hippocampal CA1 neurons of the mice;Immunohistochemical staining techniques to examine the expression of NF-κB in hippocampus.Using WB to detect the BDNF expression in hippocampal tissue and Elisa to detect the expression of TNF-α,IL-1β,and IL-6 in mouse brain tissue.Results The body weight of mice in the MDD+DCA group was apparently higher than that in the MDD group.The glucose preference rate of mice in the MDD+DCA group was significantly higher than that in the MDD group.The results of forced swimming and tail suspension experiments all showed that the stationary time of mice in the MDD+DCA group was significantly shorter than that in the MDD group.HE staining and immunohistochemical staining demonstrated that DCA significantly mitigated the pathological alterations in CA1 neurons of MDD mice induced by chronic unpredictable mild stress,along with a decrease in the expression of NF-κB.The expression of brain-derived neurotrophic factor (BDNF) protein of MDD+DCA group mice were obviously higher than that in the MDD group.The levels of TNF-α,IL-1 β,and IL-6 in the brain tissue of mice in the MDD+DCA group were apparently lower than in the MDD group.Conclusion Therefore,we conclude that DCA promotes neural repair and significantly improves depression behaviors through such mechanisms,which we propose with experimental evidence,that DCA increases BDNF expression in the hippocampal tissue and further reduces inflammation in the brain tissue of mice with depr

关 键 词：中药合剂 地龙 抑郁症 炎症因子 神经营养因子 

分 类 号：R749.4[医药卫生—神经病学与精神病学] R363[医药卫生—临床医学]