Screening for Bioactive Compounds from Mulberry Leaves Combining Bio-Affinity Ultrafiltration withα-Glucosidase and Pancreatic Lipase  

作　　者：Hui Zhang Guilin Chen Yongli Zhang Felix Wambua Muema Jun Ding Mingquan Guo 

机构地区：[1]CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture,Wuhan Botanical Garden,Chinese Academy of Sciences,Wuhan,430074,China [2]Institute of Biomedical Engineering,Ningbo Institute of Materials Technology and Engineering,Chinese Academy of Sciences,Ningbo,315201,China [3]University of Chinese Academy of Sciences,Beijing,100049,China [4]Ningbo Cixi Institute of Biomedical Engineering,Ningbo,315300,China

出　　处：《Journal of Analysis and Testing》2024年第3期315-326,共12页分析检测（英文）

摘　　要：With the growing prevalence of diabetes and obesity worldwide,the role ofα-glucosidase and lipase inhibitors has gained increasing concentration.Mulberry,a functional medicinal and edible plant,has been reported extensively in alleviating both diabetes and obesity.However,there is still a lack of refined research on its active ingredients and mechanisms.In our study,the inhibitory activities of mulberry leaf againstα-glucosidase and pancreatic lipase reached 191.37±14.46 and 775±68.60μg/mL,respectively.Meanwhile,it also promoted glucose consumption in the HepG2 cells model.Subsequently,fourα-glucosidase ligands and four pancreatic lipase ligands were identified through bio-affinity ultrafiltration combined with liquid-chromatography-mass spectrometry(UF-HPLC–MS/MS).In vitro inhibition assays confirmed the inhibitory potential of these representative ligands.Molecular docking suggested that these possible ligands could be inserted into the active pocket of the target enzyme through multiple intermolecular forces.Our findings indicated that the polyphenols in mulberry leaves may contribute significantly to their hypoglycemic and hypolipidemic properties.Furthermore,our study provides valuable insights for the screening of active ingredients in other medicinal and edible plants,which could help in the development of new therapies for diabetes and obesity.

关 键 词：Mulberry leaves Bio-affinity ultrafiltration DIABETES OBESITY 

分 类 号：TQ28[化学工程—有机化工]