Fe_(3)O_(4)@SiO_(2)-Protein A-oHSV/CD63 Ab for Capturing Virus and Exosomes  

作　　者：Wenqing Zheng Pingyi Zheng Ran Zhao Xinyu Xu Xiao Zhang Xiaoqian Yuan Ying Xu Zichuan Liu Youxin Li 

机构地区：[1]Tianjin Key Laboratory for Modern Drug Delivery and High-Efficiency,Collaborative Innovation Center of Chemical Science and Engineering,School of Pharmaceutical Science and Technology,Faculty of Medicine,Tianjin University,Tianjin,300072,China

出　　处：《Journal of Analysis and Testing》2024年第3期335-350,共16页分析检测（英文）

基　　金：the funding supports from National Natural Science Foundation of China(21605112).

摘　　要：Protein A modified magnetic spheres(Fe_(3)O_(4)@SiO_(2)-Protein A)with 22.7 emu/g saturation magnetization were prepared and characterized.These spheres had a diameter of 10 nm and was stable up to 229.2℃,and can bond 19.694 mg/g of Protein A.The Fe_(3)O_(4)@SiO_(2)-Protein A was firstly combined with oHSV-BJ-2-A antibody to capture oHSV from disease venom,and the amount of binding virus was 1.92×10^(7)to 3.44×10^(7)PFU/g.Fe_(3)O_(4)@SiO_(2)-Protein A was modified with CD63 antibody,which was further fixed using dissuccinimide octylate(DSS)as a crosslinking agent.The prepared Fe_(3)O_(4)@SiO_(2)-Protein A-CD63 Ab was useful in isolating and enriching exosomes from cell supernatant.Compared with commercial kits,Fe_(3)O_(4)@SiO_(2)-Protein A-CD63 Ab demonstrated a better purification effect,which was successfully monitored using capillary electrophoresis.The total content of exosomes protein extracted using Fe_(3)O_(4)@SiO_(2)-Protein A-CD63 was 3.4 mg/g,which was obviously higher than that reported in some studies.In addition,Fe_(3)O_(4)@SiO_(2)-Protein A-CD63 Ab also showed its repeatability through bonding and elution of 5 cycles,which was effective in cost saving.These indicated Fe_(3)O_(4)@SiO_(2)-Protein A-CD63 Ab had the potential for large-scale purification of exosomes in practical applications.

关 键 词：Immunomagnetic spheres ANTIBODY EXOSOME Herpes simplex virus 

分 类 号：TQ460.1[化学工程—制药化工]