血清circSHKBP1、SOX3 mRNA表达与晚期胃癌患者临床病理特征及化疗疗效的关系  

The relationship between serum circSHKBP1,SOX3 and clinicopathological features and chemotherapy efficacy in patients with advanced gastric cancer

作　　者：刘鑫佳 王凯瑞 杨光 杨静 张燕 Liu Xinjia;Wang Kairui;Yang Guang;Yang Jing;Zhang Yan(Department of Oncology,Tangdu Hospital of Air Force Medical University,Shaanxi Province,Xi'an 710032,China)

机构地区：[1]空军军医大学唐都医院肿瘤科,西安710032

出　　处：《疑难病杂志》2024年第12期1460-1465,共6页Chinese Journal of Difficult and Complicated Cases

基　　金：陕西省社会发展科技攻关项目(2021SF-078)。

摘　　要：目的研究环状RNA SH3结构域激酶结合蛋白1(circSHKBP1)、SRY-box转录因子3(SOX3)mRNA表达与晚期胃癌患者化疗疗效和预后的关系。方法选取2019年1月—2021年2月空军军医大学唐都医院肿瘤科收治的晚期胃癌患者104例为研究组,根据化疗疗效分为化疗敏感亚组64例和化疗抵抗亚组40例;选取同期健康体检者50例为健康对照组。采用实时荧光定量PCR检测各组血清circSHKBP1、SOX3 mRNA水平;分析血清circSHKBP1、SOX3 mRNA表达与晚期胃癌患者临床病理特征的关系;多因素Logistic回归分析影响化疗疗效的因素;受试者工作特征(ROC)曲线分析血清circSHKBP1、SOX3 mRNA对晚期胃癌患者化疗疗效的评估价值。结果研究组血清circSHKBP1、SOX3 mRNA表达高于健康对照组(t/P=65.118/<0.001、51.471/<0.001)。肿瘤分期Ⅳ期、低分化晚期胃癌患者血清circSHKBP1、SOX3 mRNA表达高于肿瘤分期ⅢB期、高中分化的患者(circSHKBP1:t/P=37.283/<0.001、31.545/<0.001;SOX3:t/P=19.169/<0.001、26.334/<0.001)。化疗抵抗亚组肿瘤分期Ⅳ期百分比,血清circSHKBP1、SOX3 mRNA表达高于化疗敏感亚组(t/P=10.037/0.002,48.609/<0.001,26.775/<0.001)。肿瘤分期Ⅳ期及血清circSHKBP1、SOX3 mRNA高表达是影响晚期胃癌化疗抵抗的危险因素[OR(95%CI)=1.594(1.252~2.028),1.306(1.144~1.492),1.603(1.190~2.160)]。circSHKBP1、SOX3 mRNA及二者联合预测晚期胃癌患者化疗疗效的AUC分别为0.844、0.851、0.922,二者联合优于各自单独预测效能(Z/P=4.745/<0.001、4.310/<0.001)。结论晚期胃癌患者血清circSHKBP1、SOX3 mRNA表达升高,且均与肿瘤分化程度,肿瘤分期及化疗疗效有关,两者联合对晚期胃癌患者化疗疗效有较好的评估价值。Objective To study the relationship between circular RNA SH3 binding protein 1(circSHKBP1),SRY-box transcription factor 3(SOX3)and chemotherapy efficacy and prognosis in patients with advanced gastric cancer.Methods A total of 104 patients with advanced gastric cancer admitted to the Department of Oncology,Tangdu Hospital of Air Force Medical University from January 2019 to February 2021 were selected as the study group,and they were divided into chemotherapy-sensitive subgroup and chemotherapy-resistant subgroup according to the efficacy of chemotherapy;50 healthy subjects were selected as healthy control group.Real-time fluorescence quantitative PCR was used to detect the levels of serum circSHKBP1 and SOX3 mRNA in each group;the relationship between serum circSHKBP1,SOX3 mRNA expression and clinicopathological features of patients with advanced gastric cancer was analyzed.Multivariate Logistic regression analysis was used to analyze the factors affecting the efficacy of chemotherapy.The receiver operating characteristic curve was used to analyze the value of serum circSHKBP1 and SOX3 mRNA in evaluating the efficacy of chemotherapy in patients with advanced gastric cancer.Results The relative expression levels of serum circSHKBP1 and SOX3 mRNA in the study group were(3.22±0.37)and(2.72±0.35),respectively,which were higher than those in the control group(0.62±0.17),(0.71±0.19),(t=65.118,51.471,both P<0.001).The relative expression levels of serum circSHKBP1 and SOX3 mRNA in patients with TNM stage Ⅳ and poorly differentiated advanced gastric cancer were higher than those in patients with TNM stage ⅢB and high and medium differentiation(t/P=37.283/<0.001,31.545/<0.001,19.169/<0.001,26.334/<0.001).The percentage of tumor stage IV in the chemotherapy-resistant subgroup and the relative expression of serum circSHKBP1 and SOX3 mRNA were higher than those in the chemotherapy-sensitive subgroup(t/P=10.037/0.002,48.609/<0.001,26.775/<0.001).Tumor stage Ⅳ,serum circSHKBP1 and SOX3 mRNA were risk factors for che

关 键 词：胃癌晚期 环状RNA SH3结构域激酶结合蛋白1 SRY-box转录因子3 化疗疗效 

分 类 号：R735.2[医药卫生—肿瘤]