以牛蛙为实验对象药物拮抗参数测定的教学实验条件构建  

Construction of teaching experimental conditions for determination of drug antagonism parameters with bullfrog as experimental object

作　　者：金悠 刘慧[2] 杨方[1] 王芳[2] 余上斌[1] JIN You;LIU Hui;YANG Fang;WANG Fang;YU Shangbin(National Demonstration Center for Experimental Teaching of Basic Medicine,Huazhong University of Science and Technology,Wuhan,Hubei 430030,China;Department of Pharmacology,School of Basic Medicine,Tongji Medical College,Huazhong University of Science and Technology,Wuhan,Hubei 430030,China)

机构地区：[1]华中科技大学国家级基础医学实验教学示范中心,武汉430030 [2]华中科技大学同济医学院基础医学院药理学系,武汉430030

出　　处：《重庆医学》2024年第23期3535-3539,共5页Chongqing Medical Journal

基　　金：华中科技大学2023年实验技术研究项目(HZKJSYJSXM2023084)。

摘　　要：目的探讨实验对象牛蛙在药效动力学实验-药物量效曲线绘制及竞争性拮抗剂pA2测定中的应用。方法以牛蛙腹直肌为标本,采用半对数摩尔浓度累积给药法,观察竞争性拮抗药筒箭毒碱对激动剂乙酰胆碱(ACh)收缩作用的影响。结果以200~250 g牛蛙(雌雄不限)的腹直肌为标本,1.3×10^(-4)~1.3×10^(-1) mol/L系列浓度的ACh溶液能够使大多数标本在第2、3次给药时引起收缩反应,且可使标本的收缩达到最大效应;0.4×10^(-3) mol/L筒箭毒碱溶液可有效拮抗ACh的作用;此外标本产生的收缩效应与实验动物为蟾蜍时的效应具有高度相关性(r>0.90)。结论牛蛙拮抗参数测定条件可满足实验要求。Objective To explore the application of bullfrog as the experimental object in pharmacokinetic experiment drug dose-effect curve drawing and competitive antagonist pA2 determination.Methods The rectus abdominis muscle of bullfrog served as the sample.The semi-logarithmic molal concentration accumulation method was used.The effect of competitive antagonist-tubocurarine on the contraction action of acetylcholine(ACh)agonist was observed.Results The rectus abdominis of bullfrog(regardless of gender)weighed 200-250 g served as the sample.The ACh solution of 1.3×10^(-4)-1.3×10^(-1) mol/L series concentrations could cause the contraction reaction of most specimens when adding the second and third doses,moreover which could make the contraction of the sample to reach the maximum effect;0.4×10^(-3) mol/L tubocurarine solution could effectively antagonize the effect of Ach;in addition,the contraction effect generated the specimen was highly correlated with the effect of bullfrog or toad as the experiment animal.Conclusion The determination conditions of bullfrog antagonistic parameters could meet the experimental requirements.

关 键 词：牛蛙 乙酰胆碱 量效曲线 筒箭毒碱 拮抗参数pA2 

分 类 号：R-4[医药卫生]