高效液相色谱法测定祛斑美白类化妆品中的间苯二酚、阿魏酸、苯乙基间苯二酚和过氧化苯甲酰  

Determination of resorcinol,ferulic acid,phenylethylresorcinol and benzoyl peroxide in freckle whitening cosmetics by high performance liquid chromatography

作　　者：杨若璇 韩晶 郑荣 彭兴盛 许勇 栾绍嵘[2] YANG Ruoxuan;HAN Jing;ZHENG Rong;PENG Xingsheng;XU Yong;LUAN Shaorong(Shanghai Institute for Food and Drug Control,National Medical Products Administration Key Laboratory for Monitoring and Evaluation of Cosmetics,Shanghai 201203,China;School of Chemistry and Molecular Engineering,East China University of Science and Technology,Shanghai 200237,China)

机构地区：[1]上海市食品药品检验研究院,国家药品监督管理局化妆品监测评价重点实验室,上海201203 [2]华东理工大学化学与分子工程学院,上海200237

出　　处：《上海预防医学》2024年第10期997-1002,共6页Shanghai Journal of Preventive Medicine

摘　　要：【目的】建立高效液相色谱法(HPLC)测定祛斑美白类化妆品中的间苯二酚、阿魏酸、苯乙基间苯二酚和过氧化苯甲酰的分析方法以期为化妆品检验方法的建立提供数据支撑,为化妆品行业的监管提供技术支持。【方法】样品经甲醇涡旋、超声提取,经离心、微孔滤膜过滤后,用SVEA C4柱(250 mm×4.6 mm,5μm)分离,以0.1%的磷酸溶液‐乙腈为流动相,梯度洗脱,流速为1.0 mL·min^(-1),紫外检测器检测,外标法定量。【结果】间苯二酚、阿魏酸、苯乙基间苯二酚、过氧化苯甲酰在试验范围内线性良好,r>0.999。方法考察了阿魏酸或苯乙基间苯二酚的不同基质的阳性加标回收率,结果回收率均在87.48%~101.00%,相对标准偏差均在3.4%~4.1%。方法也考察了空白基质加标,回收率在93.26%~107.66%,相对标准偏差在0.90%~2.90%,检出限0.0008%~0.002%。测定30批标祛斑美白类化妆品,其中6批检出苯乙基间苯二酚,1批检出阿魏酸。【结论】该HPLC法快速简便、灵敏度高,适用于同时检测市售化妆品中的间苯二酚、阿魏酸、苯乙基间苯二酚和过氧化苯甲酰。[Objective]To establish an analytical method for the determination of resorcinol,ferulic acid,phenethylresorcinol and benzoyl peroxide in freckle whitening cosmetics by high performance liquid chromatography(HPLC),to provide data support for the establishment of cosmetics inspection methods and technical support for the supervision of the cosmetics industry.[Methods]The analytes in samples were extracted by ultrasonic acetonitrile after methanol vortex,and then filtered by centrifugation and microporous filter membrane.Finally,the analytes were separated with a SVEA C4 column(250 mm×4.6 mm,5μm).The mobile phase was composed of 0.1%phosphoric acid solution-acetonitrile,and the gradient elution was applied,with a flow rate of 1.0 mL·min^(-1).The samples were detected by an ultra-violet detector and quantified by external standard method.[Results]Resorcinol,ferulic acid,phenylethylresorcinol and benzoyl peroxide showed good linearity in the experimental range with r>0.999.HPLC was used to investigate the positive spiked recoveries of ferulic acid or phenylethylresorcinol with different matrices.The results showed that the recoveries were all in the range of 87.48%to 101.00%,and the relative standard deviations were all in the range of 3.4%to 4.1%.Furthermore,HPLC also examined the blank matrix spiked with the recoveries ranged from 93.26%to 107.66%,with the relative standard deviation of 0.90%to 2.90%.The limits of detection ranged from 0.0008%to 0.002%.Among the 30 batches of standard freckle whitening cosmetics determined,6 batches of which were detected with phenethylresorcinol and 1 batch with ferulic acid.[Conclusion]HPLC is a method with rapidity,simplicity,and high sensitivity,which is suitable for the simultaneous determination of resorcinol,ferulic acid,phenethylresorcinol and benzoyl peroxide in commercially available cosmetics.

关 键 词：间苯二酚 阿魏酸 苯乙基间苯二酚 过氧化苯甲酰 高效液相色谱法 化妆品 

分 类 号：R11[医药卫生—公共卫生与预防医学] O657.7[理学—分析化学]