Extracellular vesicles loaded dual-network bioactive sealant via immunoregulation and annulus fibrosus repair for intervertebral disc herniation  

作　　者：Zetao Wang Haofei Li Huitong Luo Hao Wang Zemin Ling Dafu Chen Qi Feng Xiaodong Cao 

机构地区：[1]School of Materials Science and Engineering,South China University of Technology,Guangzhou 510641,China [2]National Engineering Research Centre for Tissue Restoration and Reconstruction,Guangzhou 510006,China [3]School of Medicine,South China University of Technology,Guangzhou 510006,China [4]Key Laboratory of Biomedical Engineering of Guangdong Province,South China University of Technology,Guangzhou 510006,China [5]Key Laboratory of Biomedical Materials and Engineering of the Ministry of Education,South China University of Technology,Guangzhou 510006,China [6]Zhongshan Institute of Modern Industrial Technology of SCUT,Guangdong 528437,China [7]Guangdong Provincial Key Laboratory of Orthopedics and Traumatology,Department of Spinal Surgery,The First Affiliated Hospital of Sun Yat-sen University,Guangzhou 510080,China [8]Laboratory of Bone Tissue Engineering,Beijing Laboratory of Biomedical Materials,Beijing Research Institute of Orthopaedics and Traumatology,Beijing JiShuiTan Hospital,Beijing 100035,China

出　　处：《Journal of Materials Science & Technology》2024年第17期75-87,共13页材料科学技术（英文版）

基　　金：supported by the National Natural Science Foundation of China(Grant Nos.51873069,52272276,52073103,52203164);the Zhongshan Innovation Project of high-end Scientific Research Institutions(Grant No.2020AG020);the Key-Area Research and Development Program of Guangdong Province(No.2020B090924004);Beijing Municipal Health Commission(Grant Nos.BMHC-2018-4,BMHC-2019-9,PXM2020026275000002);the Postdoctoral Research Foundation of China(No.2022M711183).

摘　　要：Intervertebral disc herniation(IVDH)is a common manifestation of intervertebral disc degeneration(IVDD)characterized by inflammation that results in the rupture of the annulus fibrosus(AF)and her-niation of the nucleus pulposus(NP).While current clinical research primarily focuses on regulating the degenerative NP,the crucial role of the AF in maintaining the mechanical stability and metabolic balance of the intervertebral disc(IVD)has been overlooked.Resolving immunoregulation and AF repair is im-perative to effectively prevent recurrent herniation.Therefore,this study introduces a bioactive sealant(OD/GM/QCS-sEVs),which combines gelatin methacryloyl(GM)and oxidized dextran(OD)with quater-nized chitosan(QCS)and incorporates small extracellular vesicles(sEVs).The developed sealant possesses injectability,self-healing capabilities,tissue adhesiveness,and mechanical stability,with an average ad-hesive strength of 109.63 kPa.In vitro experiments demonstrate that OD/GM/QCS-sEVs effectively seal AF defects while preserving mechanical properties comparable to those of a normal IVD.Additionally,the sealant releases sEVs through a pH-responsive mechanism,thereby modulating macrophage polarization to the M2 phenotype via the NF-κB signaling pathway.This mechanism facilitates immunoregulation and anti-inflammatory effects,and promotes stem cell differentiation into fibrocartilage.Animal experiments confirm the ability of OD/GM/QCS-sEVs to seal defects,prevent proteoglycan loss,inhibit IVDD develop-ment,and promote AF regeneration.Overall,OD/GM/QCS-sEVs hold promise as an innovative bioactive sealant for recurrent herniation by resolving immunoregulation and AF regeneration.

关 键 词：Intervertebral disc herniation Extracellular vesicles Double-network hydrogel SEALANT IMMUNOREGULATION 

分 类 号：R318[医药卫生—生物医学工程]