S100A10通过调控NF-κB的表达对乳腺癌细胞侵袭及迁移的影响  

Effects of S100A10 on Invasion and Migration of Breast Cancer Cells by Regulating the Expression of NF-κB

作　　者：郑晓宏 邱怀鹏 袁园 黄惠玲 魏开鹏 张少波 ZHENG Xiaohong;QIU Huaipeng;YUAN Yuan;HUANG Huiling;WEI Kaipeng;ZHANG Shaobo(Department of Thyroid and Breast Surgery,NO.910 Hospital of the Joint Logistic Support Force,Quanzhou Fujian 362000,Chin)

机构地区：[1]联勤保障部队910医院甲乳外科,福建泉州362000 [2]联勤保障部队910医院门诊部,福建泉州362000 [3]联勤保障部队910医院病理科,福建泉州362000

出　　处：《联勤军事医学》2024年第10期832-836,共5页Military Medicine of Joint Logistics

基　　金：联勤保障部队910医院甲乳外科,福建泉州362000。

摘　　要：目的分析钙结合蛋白A10(S100 calcium-binding protein A10,S100A10)对小鼠乳腺癌4T1细胞株侵袭及迁移的影响,并探讨其可能的作用机制。方法小鼠乳腺癌4T1细胞株分为稳转组(慢病毒感染构建稳定过表达S100A10)、对照组(感染空白对照病毒)。采用细胞计数试剂盒(cell counting kit 8,CCK-8)检测细胞增殖能力,Annexin V-异硫氰酸荧光素(fluorescein isothiocyanate,FITC)染色法观察细胞凋亡的情况,细胞划痕实验检测细胞迁移能力,Transwell小室检测细胞侵袭能力。采用Western blot法测定S100A10蛋白、核因子κB(nuclear factor kappa B,NF-κB)相关蛋白p-P65、P65、p-P50、P50的相对表达量。采用逆转录聚合酶链反应(reverse transcription polymerase chain reaction,RT-PCR)检测S100A10 mRNA表达情况。结果稳转组小鼠的S100A10蛋白以及mRNA表达水平高于对照组(P均<0.05)。稳转组与对照组小鼠细胞增殖率随培养时间增加而增加(P均<0.05),转染后孵育2~4天稳转组小鼠细胞增殖率高于对照组(P均<0.05)。稳转组小鼠细胞凋亡率低于对照组(P<0.05),而细胞侵袭数、细胞迁移率以及NF-κB相关蛋白p-P65/P65、p-P50/P50比值均高于对照组(P均<0.05)。结论S100A10可促进小鼠乳腺癌4T1细胞增殖、迁移、侵袭,抑制细胞凋亡,其作用机制可能与上调细胞内NF-κB活性有关。Objective To analyze the effect of S100 calcium-binding protein A10(S100A10)on the invasion and migration of 4T1 cell line of breast cancer in mice,and explore its possible mechanism.Methods Mouse breast cancer 4T1 cell lines were divided into stable transfer group(lentivirus infection building stable overexpression of S100A10)and control group(infection with blank control virus).Cell counting kit 8(CCK-8)was used to detect cell proliferation,and Annexin V-fluorescein isothiocyanate(FITC)staining was used to observe cell apoptosis,cell scratch test was used to de-tect cell migration,and Transwell cell was used to detect cell invasion.The relative expressions of S100A10 and nuclear factor kappa B(NF-κB)related protein p-P65,P65,p-P50,P50 were determined by Western blot.Reverse transcription polymerase chain reaction(RT-PCR)was used to detect the mRNA expression of S100A10.Results The protein and mRNA expression levels of S100A10 in stable transfer group were higher than those in control group(all P<0.05).The cell proliferation rate of mice in stable transfer group and control group increased with the increase of culture time(all P<0.05),and the cell proliferation rate of mice in stable transfer group was higher than that in control group after incuba-tion for 2 to 4 days after transfection(all P<0.05).The apoptosis rate of mice in stable transfer group was lower than that in control group(P<0.05),but the number of cell invasion,cell mobility and the ratio of NF-κB related protein p-P65/P65 and p-P50/P50 were higher than those in control group(all P<0.05).Conclusion S100A10 can promote the proliferation,migration and invasion of mouse breast cancer 4T1 cells,and inhibit cell apoptosis,the mechanism of S100A10 may be related to the up-regulation of NF-κB activity.

关 键 词：钙结合蛋白A10 乳腺癌 细胞侵袭 细胞迁移 生物学行为 核转录因子 

分 类 号：R737.9[医药卫生—肿瘤]