外源茉莉酸甲酯诱导脐橙果实抗青霉病与活性氧代谢的关系  

Involvement of induced resistance by methyl jasmonate to blue mold and reactive oxygen species metabolism in navel orange fruit

作　　者：陈明[1] 郑致远 王印宝 郭越 蒋海燕[1] 付永琦[1] 曾教科 陈金印[1] 向妙莲[1] CHEN Ming;ZHENG Zhiyuan;WANG Yinbao;GUO Yue;JIANG Haiyan;FU Yongqi;ZENG Jiaoke;CHEN Jinyin;XIANG Miaolian(College of Agronomy,Jiangxi Agricultural University/Jiangxi Provincial Key Laboratory of Fruit and Vegetable Storage and Preserva-tion/Collaborative Innovation Center of Postharvest Key Technology and Quality Safety of Fruits and Vegetables in Jiangxi Province,Nanchang 330045,Jiangxi,China)

机构地区：[1]江西农业大学农学院·果蔬贮藏与保鲜江西省重点实验室·江西省果蔬采后处理关键技术与质量安全协同创新中心,南昌330045

出　　处：《果树学报》2024年第12期2555-2566,共12页Journal of Fruit Science

基　　金：国家自然科学基金(32260793);江西省自然科学基金(20224BAB205031);江西省柑橘产业技术体系(JXARS-07)。

摘　　要：【目的】通过探究外源茉莉酸甲酯(Methyl Jasmonate,MeJA)诱导脐橙果实采后抗青霉病与活性氧代谢的关系,为MeJA应用于控制果实采后病害提供理论依据。【方法】以纽荷尔和龙回红脐橙果实为试材,在温度(26±1)℃、湿度85%~95%的环境下,使用浓度为50μmol·L^(-1) MeJA密闭熏蒸处理24 h后,接种青霉病菌Penicillium italicum(1.25×10^(6) spores·mL^(-1))20µL,以无菌水处理为对照,另设100μmol·L^(-1)水杨苷异羟肟酸(salicyhydroxamic acid,SHAM)喷施处理,测定脐橙果实活性氧代谢相关指标并分析关键酶基因表达水平。【结果】50μmol·L^(-1) MeJA熏蒸处理显著提高2个脐橙品种果实的谷胱甘肽还原酶(GR)、抗坏血酸过氧化物酶(APX)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、单脱氢抗坏血酸还原酶(MDHAR)和脱氢抗坏血酸还原酶(DHAR)活性以及对应酶基因CsGR、CsAPX、Cu-ZnSOD、CsCAT、CsMDHAR、CsDHAR3的相对表达量,在纽荷尔中MeJA处理分别为对照的1.10、1.16、1.12、1.69、1.21、1.43、1.16、1.42、1.56、1.36、1.58、2.32倍,在龙回红中MeJA处理分别为对照的1.13、1.49、1.02、1.41、1.26、1.79、2.98、1.59、1.10、1.79、1.57、1.31倍;同时,显著提高了还原型谷胱甘肽(GSH)、抗坏血酸(AsA)含量和DPPH自由基清除率,降低了过氧化氢(H2O2)含量和超氧阴离子(O2.-)产生速率,延缓了丙二醛(MDA)含量的积累。【结论】使用MeJA于接种前24 h熏蒸脐橙果实,能提高柑橘果实活性氧代谢相关酶活性以及关键酶基因的表达量,增强果实对青霉病的抗性。【Objective】Navel orange[Citrus sinensis(Linn.)Osbeck],one of the most widely planted citrus fruit trees in the world,is mainly planted in Chongqing,Hubei,Jiangxi,Fujian and other places in southern China.However,navel orange fruits are susceptible to diseases caused by pathogens during postharvest transportation and storage,seriously affecting the quality and economic value of the fruits.The blue mold caused by Penicillium italicum is one of the most important postharvest diseases in navel orange fruits,which is currently mainly controlled by chemicals.Currently there is an urgent need to de-velop safer and more efficient preservation techniques to control post-harvest diseases of navel orange fruits.Methyl jasmonate(MeJA)is an important natural plant growth regulator,which can be used as an inducer of resistance to pathogen infestation to produce a defense response in plants.In recent years,the aspect of MeJA-induced disease resistance in plants has become a hot issue,but there are fewer reports on MeJA-induced resistance to P.italicum in navel orange fruits.In this study,we investigated the rela-tionship between exogenous MeJA-induced postharvest resistance to the blue mold and reactive oxygen metabolism in navel orange fruits to provide a theoretical basis for the potential of the application of MeJA to control postharvest fruit diseases.【Methods】In this study,Newhall and Longhuihong navel orange fruits were used as test materials.Firstly,the fruits were cleaned with 0.05%sodium hypochlorite and water,then after being dried at room temperature,one group fruits were treated with 50μmol·L^(-1) MeJA for 24 h at(26±1)℃and 85%-95%relative humidity,another group fruits were sprayed with 100μmol·L^(-1) SHAM,and the third group treated with sterile water was used as control.The conidia of P.italicum that had been cultured on PDA medium for one week were collected and made into a spore suspension with a concentration of 1.25×10^(6) spores·mL^(-1).The surface of the fruits was sterilized with 75%ethanol

关 键 词：脐橙果实 茉莉酸甲酯 青霉病抗性 活性氧代谢 

分 类 号：S666.4[农业科学—果树学]