参芪扶正注射液联合顺铂协同抑制非小细胞肺癌增殖  

Study on the synergistic inhibition of proliferation of non-small cell lung cancer by Shenqi Fuzheng injection combined with cisplatin

作　　者：郑宇 董爽 胡祖成 闫丽文 彭凤栖 王浩捷 杜守颖[2] 陆洋[2] ZHENG Yu;DONG Shuang;HU Zucheng;YAN Liwen;PENG Fengqi;WANG Haojie;DU Shouying;LU Yang(Department of Traditional Chinese Medicine,Beijing Nuclear Industry Hospital,Beijing 100045,China;School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 102488,China)

机构地区：[1]北京核工业医院中医科,北京100045 [2]北京中医药大学中药学院,北京102488

出　　处：《长春中医药大学学报》2024年第12期1335-1341,共7页Journal of Changchun University of Chinese Medicine

基　　金：国家自然科学基金面上项目(82173989)。

摘　　要：目的探讨参芪扶正注射液联合顺铂(DDP)抑制非小细胞肺癌(NSCLC)增值效果。方法采用MTT比色法和流式细胞术测定A549/LLC细胞的抑制作用和凋亡;通过免疫荧光评估形态学,通过蛋白质印迹法检测细胞凋亡相关蛋白,皮下荷瘤小鼠模型评价抗肿瘤活性。结果参芪扶正注射液对肿瘤细胞没有抑制作用,但与顺铂联合使用在体外和体内均显示出协同或加强抗肿瘤作用。免疫荧光结果显示,参芪扶正注射液联合顺铂可增强顺铂对肿瘤细胞膜的破坏作用。在体内,参芪扶正注射液和顺铂处理后荷瘤小鼠肿瘤权重显著降低,胸腺/脾脏指数、IL-2升高。进一步的研究表明,上述抗癌作用与Caspase家族、Bax/Bcl-2表达上调以及抑制核因子-κB(NF-κB)、血管内皮生长因子(VEGF)通路有关。结论参芪扶正注射液可提高顺铂疗效,提高机体免疫力。Objective To explore the effect of the synergistic inhibition of proliferation of non-small cell lung cancer(NSCLC)by Shenqi Fuzheng(SQFZ)injection combined with cisplatin(DDP).Methods MTT colorimetric method and flow cytometry were used to determined the inhibitory effect and apoptosis of A549/LLC cells.Morphology was assessed by immunofluorescence.Apoptosis related proteins were detected by Western blot.Anti-tumor activity was determined by subcutaneous tumorbearing mouse model.Results SQFZ injection had no inhibitory effect on tumor cells,but when used in combination with DDP,it showed synergistic or enhanced anti-tumor effects both in vitro and in vivo.Immunofluorescence results showed that combination of SQFZ and DDP could enhance the destructive effect of DDP on tumor cell membrane.In vivo,tumor weights of tumor-bearing mice significantly decreased,and thymus/spleen index and the level of interleukin-2(IL-2)increased after treatment with SQFZ and DDP.Further study indicated that the anticancer effects mentioned above were associated with upregulation expression of Caspase family and Bax/Bcl-2,and inhibition of nuclear factor kappa-B(NF-κB)and vascular endothelial growth factor(VEGF)pathways.Conclusion SQFZ injection can improve the efficacy of DDP and enhance the immunity of the body.

关 键 词：顺铂 联合治疗 肺癌 参芪扶正注射液 协同效应 

分 类 号：R73[医药卫生—肿瘤]