葡萄牙栖盐田菌LLJ914的全基因组测序和比较基因组分析  

Genome Sequencing and Comparative Genomic Analysis of Salinicola lusitanus LLJ914

作　　者：李娟娟 刘子涵 王雅楠 刘莉君 张晓华[1,2,3] 于敏 Li Juanjuan;Liu Zihan;Wang Yanan;Liu Lijun;Zhang Xiaohua;Yu Min(College of Marine Life Sciences,Ocean University of China,Qingdao 266003,China;Laboratory for Marine Ecology and Environmental Science,Qingdao Marine Science and Technology Center,Qingdao 266237,China;Institute of Evolution&Marine Biodiversity,Ocean University of China,Qingdao 266003,China)

机构地区：[1]中国海洋大学海洋生命学院,山东青岛266003 [2]青岛海洋科技中心海洋生态与环境科学功能实验室,山东青岛266237 [3]中国海洋大学进化与海洋生物多样性研究所,山东青岛266003

出　　处：《中国海洋大学学报（自然科学版）》2025年第1期51-65,共15页Periodical of Ocean University of China

基　　金：国家自然科学基金项目(42376145,41976137)资助。

摘　　要：栖盐田菌属细菌因具有较强渗透压耐受能力而备受关注。然而,目前对其适应高盐或其他海洋极端环境的机制尚未探明。为揭示分离自冲绳海槽热液区的葡萄牙栖盐田菌(Salinicola lusitanus)LLJ914与其同属菌株之间的遗传特征及代谢潜力的差异,探究其在极端海洋生境下的适应机制。通过全基因组测序获得葡萄牙栖盐田菌LLJ914全基因组序列,选取同属其他菌株的基因组进行比较基因组学分析,探究栖盐田菌属各菌株及不同环境来源的葡萄牙栖盐田菌代谢潜力的差异。菌株LLJ914基因组大小为4781556 bp,GC含量为64.0%,共编码4229个蛋白,69个tRNA,12个rRNA。通过构建系统发育树,并进行平均核苷酸和平均氨基酸一致性分析,发现该菌株与马齿苋(Halimione portulacoides)内共生的葡萄牙栖盐田菌CR50^(T)亲缘关系最近。通过功能基因注释分析,发现葡萄牙栖盐田菌具有抵抗各种重金属的相关基因和利用甲基膦酸产生甲烷的phn基因簇;相比于植物内共生菌CR50^(T),菌株LLJ914基因组中包含更多与氨基酸和碳水化合物的运输代谢、能量生产和转换以及转录相关的功能基因。此外,菌株LLJ914基因组中还包含特有的与重金属抵抗、免疫防御及有氧呼吸相关的基因,这可能与其适应复杂极端的深海热液环境有关。本研究揭示了葡萄牙栖盐田菌LLJ914适应深海热液环境的遗传特征及代谢潜力,为更好地认识热液微生物的生态功能提供了参考。Salinicola has attracted much attention due to its strong osmotic pressure tolerance.However,the mechanism by which it adapts to high salt or other extreme marine environments is not yet known.To elucidate the differences of genetic characteristics and metabolic potential between Salinicola lusitanus LLJ914 isolated from the hydrothermal field of Okinawa Trough and bacteria in the same genus,exploring the adaptive mechanism of strain LLJ914 in extreme marine habitats,the complete genome sequence of S.lusitanus LLJ914 was obtained by whole genome sequencing.Genomes of bacteria belonging to the same genus were selected for comparative genomic analysis.The differences in metabolic potential of the genus Salinicola and S.lusitanus strains isolated from different environments were explored.The genome size of strain LLJ914 is 4781556 bp with a G+C content of 64.0%.It encodes a total of 4229 proteins,69 tRNAs and 12 rRNAs.By constructing phylogenetic trees and performing average nucleotide and average amino acid consistency analyses,it was found that strain LLJ914 was most closely related to S.lusitanus CR50^(T) from halophyte Halimione portulacoide.According to the annotation of functional genes,bacteria belonging to S.lusitanus possess various genes associated with heavy metal resistance and methanogen through methylphosphonic acid(the gene cluster phn).Compared with strain CR50^(T),the genome of strain LLJ914 contained more genes related to amino acid and carbohydrate transport metabolism,energy production and conversion,and transcription.In addition,strain LLJ914 also harbored unique genes associated with heavy metal resistance,immune defense and aerobic respiration,which may contribute to its adaptation to the complex and extreme deep-sea hydrothermal environment.In this study,the genetic characteristics and metabolic potential of S.lusitanus LLJ914 were revealed,which should aid to understand its adaption to deep-sea hydrothermal environments.These findings provided a reference to better understanding the ecologic

关 键 词：葡萄牙栖盐田菌 极端环境 热液区 冲绳海槽 全基因组测序 比较基因组分析 

分 类 号：Q938.2[生物学—微生物学]