香菇多糖对小鼠心肌肥厚伴急性缺血的改善作用  

Effects of lentinan on improving myocardial hypertrophy with acute myocardial ischemia in mice

作　　者：张小婷 古丽娅尔·吾麦尔 张旭 王梦伟 许旭 高建平[1] ZHANG Xiaoting;WUMAIER Guliar;ZHANG Xu;WANG Mengwei;XU Xu;GAO Jianping(School of Pharmacy,Shanghai University of TCM,Shanghai,201203 P.R.China;School of Chemical and Environmental Engineering,Shanghai Institute of Technology,Shanghai,201418 P.R.China)

机构地区：[1]上海中医药大学中药学院,上海201203 [2]上海应用技术大学化学与环境工程学院,上海201418

出　　处：《华西药学杂志》2024年第6期664-668,共5页West China Journal of Pharmaceutical Sciences

基　　金：国家自然科学基金资助项目(批准号:82074055);上海中医药大学预算内项目(2020LK016)。

摘　　要：目的研究香菇多糖对小鼠心肌肥厚伴急性缺血的作用。方法sc小鼠2 mg·kg^(-1)异丙肾上腺素,持续14 d,最后2次sc 50 mg·kg^(-1),诱导心肌肥厚伴急性缺血小鼠模型。造模同时,ig 25 mg·kg^(-1)香菇多糖,持续16 d,每天1次。记录心电图;检测血清中心肌酶谱和肾功能指标;分别称量脏器重量,计算对应脏器指数;心尖HE染色进行病理组织学检查;测定心肌组织中的能量物质水平。结果与模型组比较,香菇多糖可改善小鼠心电图的异常,逆转心脏、脾脏、肺脏和胸腺各脏器指数异常、改善心肌酶谱和肾功能指标的异常,降低心脏病理改变,提高磷酸肌酸的水平。结论香菇多糖对心肌肥厚伴急性心肌缺血有较好的防治作用,其作用可能与改善心肌能量物质储备有关。OBJECTIVE To study the effects of lentinan on myocardial hypertrophy with acute ischemia in mice.METHODS A mice model of myocardial hypertrophy with acute ischemia was established by administering isoproterenol subcutaneously at a dose of 2 mg·kg^(-1)per day for 14 days,followed by two additional subcutaneous injections of 50 mg·kg^(-1)per day.Concurrently,the mice were given 25 mg·kg^(-1)lentinan once a day by gavage for 16 days.After administration,electrocardiograms were recorded,and serum myocardial enzyme profile and renal function indexes were detected.The organs were weighed individually,and the corresponding organ indices were calculated.HE staining of the heart apex was used for histopathological examination.Levels of energy substances in myocardial tissues were measured.RESULTS Compared to the model group,lentinan improved the abnormalities of electrocardiogram,reversed the abnormalities of heart,spleen,lung,and thymus indices,improved the abnormalities of myocardial enzyme profiles and renal function indices,reduced cardiac pathological changes,and increased creatine phosphate level.CONCLUSION Lentinan has good preventive and therapeutic effects on myocardial hypertrophy associated with acute myocardial ischemia,which may be related to the improvement of myocardial energy material reserves.

关 键 词：心肌肥厚 心肌缺血 异丙肾上腺素 香菇多糖 心电图 心肌酶谱 能量代谢 磷酸肌酸 

分 类 号：R96[医药卫生—药理学]