蒲公英根提取物对乳腺癌细胞MCF-7恶性生物学行为及蛋白激酶B/低氧诱导因子-1α通路的影响  

Effects of dandelion root extract on the malignant behaviors of the breast cancer cell line MCF-7 and the Akt/HIF-1αsignaling pathway

作　　者：王开裕 邢明远 许海丹 WANG Kaiyu;XING Mingyuan;XU Haidan(Department of Pharmacy,Guangdong Provincial Hospital of Chinese Medicine,Hainan Hospital,Haikou,Hainan 570203;Department of Oncology,Guangdong Provincial Hospital of Chinese Medicine,Hainan Hospital,Haikou,Hainan 570203)

机构地区：[1]广东省中医院海南医院药学部,海南海口570203 [2]广东省中医院海南医院肿瘤科,海南海口570203

出　　处：《河北中医》2024年第12期2024-2028,共5页Hebei Journal of Traditional Chinese Medicine

摘　　要：目的研究蒲公英根提取物对乳腺癌细胞MCF-7迁移、侵袭、增殖及蛋白激酶B/低氧诱导因子-1α(Akt/HIF-1α)通路的影响。方法通过体外培养人乳腺癌细胞MCF-7,并分为DMSO对照组及蒲公英根提取物25、50、100、200、400μg/mL组,DMSO对照组以含2 mL DMSO的培养基处理,其余各组以含有对应浓度的蒲公英根提取物2 mL的培养基处理。划痕实验检测细胞迁移能力,Transwell小室实验检测细胞侵袭情况,噻唑蓝(MTT)法检测细胞活力,Western blot法检测Akt/HIF-1α通路蛋白表达。结果划痕实验结果显示,各组细胞12、24 h愈合率变化趋势一致,蒲公英根提取物各浓度组划痕愈合率均低于DMSO对照组(P<0.05),且随着蒲公英根提取物浓度的增加,12、24 h划痕愈合率逐渐降低(P<0.05);各组24 h划痕愈合率均明显高于12 h(P<0.05)。Transwell小室实验结果显示,蒲公英根提取物各浓度组细胞侵袭数量均少于DMSO对照组(P<0.05),且细胞侵袭数量随着蒲公英根提取物浓度的增加逐渐降低(P<0.05),当浓度为400μg/mL时细胞侵袭数量不再改变(P>0.05)。MTT法检测结果显示,12 h时各组细胞相对活力比较差异无统计学意义(P>0.05);24 h时蒲公英根提取物各浓度组细胞相对活力均低于DMSO对照组(P<0.05),且随着蒲公英提取物浓度的增高细胞相对活力逐渐降低(P<0.05),且24 h时蒲公英根提取物各浓度组细胞相对活力均低于12 h(P<0.05)。Western blot结果显示,与DMSO对照组相比,蒲公英根提取物各浓度组磷酸化Akt(p-Akt)、HIF-1α蛋白表达均降低(P<0.05),且随着浓度的升高而降低(P<0.05)。结论蒲公英根提取物能够下调Akt/HIF-1α通路表达而抑制乳腺癌MCF-7细胞的恶性生物学行为。Objective To investigate the effect of dandelion root extract on the migration,invasion,proliferation and the Thr protein kinase B(Akt)/hypoxia-inducible factor-1a(HIF-1α)signaling pathway in the breast cancer cell line MCF-7.Methods Human breast cancer cell line MCF-7 was cultured in vitro,and induced with DMSO(negative control)and dandelion root extract at varying concentrations(25,50,100,200 and 400μg/mL).Cells were cultured in 2 mL of fresh medium containing DMSO or dandelion root extract.Cell migration,invasion and viability were detected by wound healing assay,Transwell assay and MTT assay,respectively.Expression levels of proteins involved in the Akt/HIF-1αsignaling pathway were determined by Western blot.Results The wound healing assay data showed that 12-h and 24-h wound healing rate in MCF-7 cells induced with dandelion root extract at varying concentrations were significantly reduced in a dose-dependent manner than those induced with DMSO(P<0.05),and the 24-h wound healing rate in each group was significantly higher than the 12-h value(P<0.05).Transwell assay data showed that the invasive cell number in MCF-7 cells induced with dandelion root extract at varying concentrations was significantly reduced in a dose-dependent manner than those induced with DMSO(P<0.05),which was not changed after the dose increased to 400μg/mL(P>0.05).MTT assay showed no significant difference in the 12-h viability among groups(P>0.05),but significantly lower in MCF-7 cells induced with dandelion root extract at varying concentrations in a dose-dependent manner(P<0.05).The 24-h viability in each group was significantly lower than the 12-h value(P<0.05).Western blot showed significantly downregulated p-Akt and HIF-1αin MCF-7 cells induced with dandelion root extract at varying concentrations in a dose-dependent manner(P<0.05).Conclusion Dandelion root extract inhibits the malignant biological behaviors of breast cancer MCF-7 cells by inhibiting the Akt/HIF-1αsignaling pathway.

关 键 词：乳腺肿瘤 蒲公英 细胞生物学 

分 类 号：R737.9[医药卫生—肿瘤] R282[医药卫生—临床医学] R-33