LINC01140过表达对肺腺癌细胞H1975增殖和侵袭的影响  

Impact of LINC01140 Overexpression on Proliferation and Invasion of lung Adenocarcinoma Cell H1975

作　　者：崔玉洁 张洁[1] 张立晓 魏亚红 刘苗苗[1] 陈连刚 CUI Yujie;ZHANG Jie;ZHANG Lixiao;WEI Yahong;LIU Miaomiao;CHEN Liangang(Department of Oncology,Hebei People's Hospital,Shijiazhuang 050000,China;Department of Oncology,the 980th Hospital of the Joint Logistics Support Force of the PLA,Shijiazhuang 050082,China)

机构地区：[1]河北省人民医院肿瘤科,石家庄050000 [2]联勤保障部队第九八〇医院肿瘤科,石家庄050082

出　　处：《临床误诊误治》2024年第21期73-78,共6页Clinical Misdiagnosis & Mistherapy

基　　金：河北省青年科技计划项目(20220882);河北省政府资助临床医学优秀人才项目(ZF2025031)。

摘　　要：目的 探讨LINC01140过表达对肺腺癌细胞H1975增殖及侵袭的影响,以为靶向药物的选择和预后评估提供依据。方法 基于生物信息学结果,选取表皮生长因子受体(EGFR)T790M突变阳性的肺腺癌细胞株H1975,通过转染技术构建LINC01140过表达细胞系,分为对照组(H1975/NC)、高表达组(H1975/LINC01140),RT-PCR检测细胞系中LINC01140的表达水平。对2组细胞进行培养,用MTS检测细胞的增殖能力,平板划痕试验检测细胞迁移能力,Transwell试验检测细胞侵袭能力。结果 通过数据库得知LINC01140在肺癌组织中的表达低于癌旁组织,且与临床特征及生存均有关(P<0.05)。同时,本研究结果证实成功构建LINC01140过表达H1975细胞系。与H1975/NC比较,H1975/LINC01140中LINC01140表达水平升高,细胞吸光度、迁移率增高,穿膜细胞数增多(P<0.05)。结论 LINC01140过表达可促进肺腺癌细胞H1975的增殖、迁移和侵袭,检测其表达水平可指导临床靶向药物的选择,提高疗效,从而改善预后。Objective To investigate the impact of LINC01140 overexpression on the proliferation and invasion of lung adenocarcinoma cell H1975,so as to provide a basis for the selection of targeted drugs and prognostic evaluation.Methods Based on the results of bioinformatics,lung adenocarcinoma cell line H1975 with positive mutation of epidermal growth factor receptor(EGFR)T790M was selected and overexpressed LINC01140 cell line was constructed by transfection technique,and divided into a control group(H1975/NC)and a high expression group(H1975/LINC01140).The expression level of LINC01140 in the cell line was detected by RT-PCR.They were divided into control group(H1975/NC)and high ex-pression group(H1975/LINC01140).Cells in the two groups were cultured.The proliferation ability of cells was detected by MTS test,the migration ability of cells was detected by wound healing assay,and the invasion ability of cells was detected by Transwell test.Results The results of bioinformatics revealed that the expression of LINC01140 in lung cancer tissues was lower than that in adjacent tissues,which was related to clinical features and survival(P<005).In the meantime,the re-sults of this study confirmed the successful construction of LINC01140 overexpressed H1975 cell line.Compared with H1975/NC,the expression level of LINC01140 in H1975/LINC01140 increased,cell absorbance and migration rate increased,and the number of transmembrane cells increased(P<005).Conclusion LINC01140 overexpression promotes the prolifera-tion,migration and invasion of lung adenocarcinoma cell H1975.Detection of its expression level can guide the selection of clinical targeted drugs,improve efficacy,and thus improve prognosis.

关 键 词：肺肿瘤 LINC01140 H1975 细胞增殖 细胞运动 肿瘤侵润 

分 类 号：R734.2[医药卫生—肿瘤]