囊泡分拣蛋白35(vps35)通过EMT影响结肠癌转移  

Vesicle sorting protein 35(vps35)affects colon cancer metastasis via Wnt/β-catenin

作　　者：袁丽杰 Yuan Lijie(Xiamen Medical College,Xiamen,Fujian,361023,China)

机构地区：[1]厦门医学院,福建厦门361023

出　　处：《齐齐哈尔医学院学报》2024年第22期2112-2117,共6页Journal of Qiqihar Medical University

基　　金：福建省自然基金项目(2020D036);厦门市科技项目(No.3502Z20214ZD1331)。

摘　　要：目的研究vps35是否通过调节结肠癌上皮间质化影响结肠癌转移。方法使用GEO2R软件分析TCGA(The Cancer Genome atlas)数据库中275例结肠癌(COAD)样本和349例癌旁样本测序结果,选取经TNM分期系统确定为stageⅠ、stageⅡ、stageⅢ、stageⅣ期的组织标本(n=4)为研究对象,以4例正常结肠组织为对照,利用免疫组化技术分析vps35在结肠癌中样本中的表达情况以及与分期的关系;选取20对结肠癌和癌旁组织,提取总RNA,并利用PCR技术分析vps35在上述样本中的表达水平;使用细胞转染、免疫荧光技术分析HT29细胞中vps35与上皮间质化关键蛋白E-cadherin、vimentin的关系,利用RNA干涉、侵袭实验验证vps35对HT29细胞侵袭能力的影响。结果vps35在结肠癌组织中呈高表达并与病理分期呈正相关;沉默vps35可抑制结肠癌细胞EMT,敲减vps35可抑制HT29细胞的侵袭,敲减vps35后,细胞膜上的E-cadherin表达增多、vimentin表达下调。结论vps35是结肠癌转移的关键分子,或能成为结肠癌的治疗新靶点。Objective To study whether vps35 regulates the epithelial-mesenchymal transition in colorectal cancer to affect its metastasis.Methods The sequencing results of 275 colon cancer(COAD)samples and 349 adjacent samples from the cancer genome atlas(TCGA)database was analyzed using GEO2R software.The expression of vps35 in 20 clinical colon cancer samples was analyzed by immunohistochemistry and its relationship with staging was investigated.The expression level of vps35 in colon cancer was analyzed by PCR.The relationship between vps35 and epithelial-mesenchymal transition key proteins E-cadherin and vimentin in HT29 cells was analyzed by cell transfection,immunofluorescence technology.The effect of vps35 on the invasive ability of HT29 cells was verified by RNA interference and invasion experiment.Results Vps35 was highly expressed in colon cancer tissues and positively correlated with pathological staging.Silencing vps35 inhibited EMT in colon cancer cells.Knockdown of vps35 inhibited the invasive ability of HT29 cells.After knockdown of vps35,the expression of E-cadherin on the cell membrane increased and the expression of vimentin was downregulated.Conclusions Vps35 is a key molecule in the metastasis of colon cancer,it could be a new target for treatment of colon cancer.

关 键 词：囊泡分拣蛋白35(vps35) 结肠癌 转移 

分 类 号：R735.5[医药卫生—肿瘤]