ITGAL在头颈鳞状细胞癌中的表达与免疫学作用  

Expression and immunologic role of ITGAL in head and neck squamous cell carcinoma

作　　者：张恺 罗观发 鞠伟 钟安菁[4] 史圆圆[4] 石超文 杨细虎[2] ZHANG Kai;LUO Guanfa;JU Wei;ZHONG Anqing;SHI Yuanyuan;SHI Chaowen;YANG Xihu(Department of Otorhinolaryngology and Head and Neck Surgery,Affiliated Hospital of Jiangsu University,Zhenjiang 212000,Jiangsu,China;Department of Stomatology,Affiliated Hospital of Jiangsu University,Zhenjiang 212000,Jiangsu,China;Department of Burns and Plastic Surgery,Affiliated Hospital of Jiangsu University,Zhenjiang 212000,Jiangsu,China;Department of Pathology,Affiliated Hospital of Jiangsu University,Zhenjiang 212000,Jiangsu,China;School of Life Sciences,Jiangsu University,Zhenjiang 212013,Jiangsu,China)

机构地区：[1]江苏大学附属医院耳鼻咽喉头颈外科,镇江212000 [2]江苏大学附属医院口腔科,镇江212000 [3]江苏大学附属医院烧伤整形科,镇江212000 [4]江苏大学附属医院病理科,镇江212000 [5]江苏大学生命科学学院,镇江212013

出　　处：《医学研究与战创伤救治》2024年第10期1034-1039,共6页Journal of Medical Research & Combat Trauma Care

基　　金：国家自然科学基金(82173380)。

摘　　要：目的探究整合素αL(ITGAL)在头颈鳞状细胞癌(HNSCC)的表达及免疫学作用。方法利用公共数据库,依据ITGAL表达量中位数将患者分为ITGAL高表达组和ITGAL低表达组,分析ITGAL在HNSCC中的表达及预后价值;探讨ITGAL对免疫细胞浸润和肿瘤微环境的影响。选择2016年10月至2024年4月在江苏大学附属医院接受治疗的口腔鳞状细胞癌患者,通过免疫组织化学法验证ITGAL蛋白在癌组织及其配对癌旁组织中的差异表达。结果ITGAL mRNA和蛋白在HNSCC癌组织表达显著高于癌旁组织(P<0.01),并与患者的生存率的提高有关(P<0.05);ITGAL的上调与肿瘤组织中抗肿瘤免疫细胞(如CD8+T细胞和CD20+B细胞)的浸润增强呈正相关(P<0.000)。ITGAL的表达与趋化因子基因CXCL9、CXCL10、免疫激活基因PRF1、IFNG和检查点基因CTLA-4表达呈正相关(P<0.000);ITGAL蛋白质在癌组织中的表达[8.00(4.00~9.00)]显著高于癌旁组织[3.00(1.00~6.00)],差异具有统计学意义(P<0.000)。结论ITGAL在HNSCC组织中高表达,可能作为一种有前景的生物标志物,为抗肿瘤治疗提供了新的视角。Objective This study aims to explore the expression and immunologic role of integrinαL(ITGAL)in head and neck squamous cell carcinoma(HNSCC).Methods Using public databases,patients were divided into ITGAL high expression group and ITGAL low expression group according to the median expression of ITGAL,and the expression and prognostic value of ITGAL in HNSCC were analyzed.The effect of ITGAL on immune cell infiltration and tumor microenvironment was explored.Patients with oral squamous cell carcinoma who were treated at Jiangsu University Hospital from October 2016 to April 2024 were selected and the differential expression of ITGAL protein in cancer tissues and their paired paracancerous tissues was verified by immunohistochemistry.Results The expression of ITGAL mRNA and protein in HNSCC cancer tissues is significantly higher than that in adjacent tissues(P<0.01),and is related to the improvement of patient survival rate(P<0.05);the upregulation of ITGAL mRNA was associated with enhanced infiltration of anti-tumor immune cells(e.g.,CD8+T cells and CD20+B cells)in tumor tissues(P<0.000).The expression of ITGAL mRNA was also positively correlated with chemokine genes(CXCL9,CXCL10),immune activation genes(PRF1,IFNG)and checkpoint genes(CTLA-4)(P<0.000);the expression of ITGAL protein in cancer tissues[8.00(4.00-9.00)]was significantly higher than that in adjacent tissues[3.00(1.00-6.00)],and the difference was statistically significant(P<0.000).Conclusion ITGAL is highly expressed in HNSCC and may serve as a promising biomarker and provide new perspectives for antitumor therapy.

关 键 词：整合素αL 头颈鳞状细胞癌 肿瘤微环境 免疫细胞浸润 

分 类 号：R739.91[医药卫生—肿瘤]