Simultaneous determination of 14 nitroimidazoles using thin-layer chromatography combined with surface-enhanced Raman spectroscopy(TLC-SERS)  

作　　者：Siqian Shi Hang Yu Fangwei Yang Weirong Yao Yunfei Xie 

机构地区：[1]State Key Laboratory of Food Science and Technology,Jiangnan University,China [2]School of Food Science and Technology,Jiangnan University,China [3]Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province,Jiangnan University,No.1800 Lihu Avenue,Wuxi,214122,Jiangsu Province,China

出　　处：《Food Bioscience》2022年第4期310-319,共10页食品生物科学（英文）

基　　金：supported by National Natural Science Foundation of China(32172326);National Key R&D Program of China(2018YFC1602300,2020YFC1606800);Modern agriculture in Jiangsu Province-key and general projects(BE2019362);Science and technology planning project of Jiangsu market supervision and Administration Bureau(KJ204132,KJ21125006);Suzhou science and technology development plan(SS2019016).

摘　　要：Nitroimidazoles are a family of veterinary drugs containing a 5-nitroimidazole ring structure,which are commonly used antibiotics.In view of their potential genotoxicity and possible coexistence in actual samples,it is necessary to establish a rapid and effective method for the simultaneous determination of multiple nitroimidazoles.In this study,a rapid thin-layer chromatography combined with surface-enhanced Raman spectroscopy(TLC-SER)detection method for 14 nitroimidazole compounds has been established.The theoretical Raman spectra of the nitroimidazoles were calculated using the B3LYP/6-311+G(d)basis set in density functional theory(DFT)and the theoretical Raman spectra of TNZ,NBZ,ONZ,SCZ,DMZ,MNI,2-HMZ,4-HMZ,CMNI,IPZ,MNZ-OH and HMDMZ have been obtained for the first time.SERS detection was carried out on a gold nanoparticles(AuNPs)substrate,which exhibited strong SERS signals and a limit of detection limit of 0.1 mg/L.By optimizing the conditions used for the developing agent in TLC,12 nitroimidazoles were successfully separated,while MNZ and RNZ still could not be separated.When combined with the chemometrics analysis method,the TLC-SERS method can realize the simultaneous detection of 14 nitroimidazoles.Actual pork samples were spiked with nitroimidazoles with a recovery rate of 80%-110%and RSD of 0.74%-16.70%,which showed that SERS and TLC-SERS methods are also effective for detection in actual samples.The detection time of this method is<10 min,which is suitable for the rapid detection of nitroimidazoles,especially for the rapid qualitative identification of nitroimidazoles in situ.

关 键 词：NITROIMIDAZOLES SERS TLC-SERS PCA 

分 类 号：TS207[轻工技术与工程—食品科学]