实验小动物超声仪3D血管多普勒超声检测新生小鼠大脑Willis环的方法学研究  

Study on the Method of Detecting the Circle of Willis in the Neonatal Mouse Brain by 3D Vascular Doppler Ultrasound with Experimental Small Animal Ultrasound Instrument

作　　者：黄松 刘丽娜 许晴 HUANG Song;LIU Lina;XU Qing(State-Owned Assets and Laboratory Management Office,Capital Medical University,Beijing 100069,China;Core Facilities Centre,Capital Medical University,Beijing 100069,China)

机构地区：[1]首都医科大学国有资产与实验室管理处,北京100069 [2]首都医科大学中心实验室,北京100069

出　　处：《中国医疗设备》2024年第12期126-130,共5页China Medical Devices

基　　金：北京市自然科学基金(5202006)。

摘　　要：目的探索应用小动物超声影像系统3D血管超声检测新生小鼠大脑Willis环的方法。方法选用生后1 d和7 d正常小鼠各6只,应用超高分辨率小动物专用超声影像系统及30 MHz探头,确定显示Willis环的探测径路和方向。通过完整的颅骨和皮肤对新生小鼠大脑进行3D多普勒断层扫描,以显示大脑Willis环;同时检测新生小鼠大脑中动脉(Middle Cerebral Artery,MCA)的血流信号,测量舒张末期血流速度(End Diastolic Velocity,EDV)、收缩期峰值流速(Peak Systolic Velocity,PSV)、阻力指数(Resistive Index,RI)及变异系数(Coefficient of Variation,CV);应用灌注技术观察新生小鼠Willis环的形态结构。结果采用前囟为探测径路,探头呈45°角向下连续扫描,利用彩色多普勒血管显像结合3D重构技术,可立体显示新生小鼠Willis环,与灌注技术显示的Willis环的形态结构相一致。应用脉冲多普勒可采集到稳定的MCA的血流信号,CV为1.9%~7.9%,左右两侧MCA的血流信号EDV、PSV和RI差异均无统计学意义(P>0.05)。结论应用小动物超声系统3D血管超声无创显示新生小鼠Willis环的结构是可行的,同时可检测MCA稳定的血流信号。本研究可为了解出生后发育过程中活体小鼠Willis环结构和功能提供实验基础。Objective To explore the method of detecting the circle of Willis in the neonatal mouse brain by three-dimensional(3D)vascular ultrasound technology of small animals.Methods The neonatal mice with post-natal ages of 1 d(n=6)and 7 d(n=6)underwent ultrasound scan imaging using the ultra-high resolution ultrasonic imaging system at a center frequency of 30 MHz,and the detection path and direction of the circle of Willis were determined.Doppler imaging and 3D vascular ultrasound of the entire mouse brain with non-invasively through the intact skull and skin were performed to display the circle of Willis in brain.At the same time,the blood flow signals of the middle cerebral artery(MCA)of newborn mice were detected,and the end diastolic velocity(EDV),peak systolic velocity(PSV),resistive index(RI)and coefficient of variation(CV)were measured.The morphology and structure of the circle of Willis in neonatal mice were observed by perfusion technique.Results With the anterior fontanelle as the detection path,the probe was continuously scanned downward at a 45°angle,and color Doppler vascular imaging combined with 3D reconstruction technology was used to display the circle of Willis of newborn mice in stereo,which was consistent with the morphology and structure of the circle of Willis shown by perfusion technique.Pulse Doppler could be used to collect stable MCA blood flow signals,CV was 1.9%~7.9%,and there were no statistical significances in EDV,PSV and RI of MCA blood flow signals on the left and right sides(P>0.05).Conclusion It is feasible to non-invasively display the structure of the circle of Willis in neonatal mouse by 3D vascular ultrasound using small animal ultrasound system,and to detect stable blood flow parameters of MCA.This study can provide an experimental basis for understanding the structure and function of the circle of Willis in living mice during postnatal development.

关 键 词：超高频超声 三维血管超声 大脑动脉环(Willis环) 大脑中动脉 新生小鼠 血流动力学 

分 类 号：R737.9[医药卫生—肿瘤]