肺鳞癌组织中TMEM42的表达及临床意义  

Expression of TMEM42 in lung squamous cell carcinoma tissues and its clinical significance

作　　者：林志明[1] 王庆 胡彦辉 LIN Zhiming;WANG Qing;HU Yanhui(Department of Geriatrics(Hematology and Oncology),Shangshui People's Hospital,Shangshui 466100,China)

机构地区：[1]商水县人民医院老年病科(血液肿瘤科),河南商水466100 [2]郑州大学附属肿瘤医院(河南省肿瘤医院)中西医结合肿瘤内科,461500

出　　处：《临床肿瘤学杂志》2024年第7期667-670,共4页Chinese Clinical Oncology

摘　　要：目的探讨跨膜蛋白42(TMEM42)在肺鳞癌(LSCC)组织中的表达及其临床意义。方法借助TGGA数据库分析TMEM42基因在LSCC组织中的表达。利用免疫组化技术和实时定量PCR法检测60对LSCC组织和癌旁组织中TMEM42的表达水平,进一步分析TMEM42表达与LSCC临床病理特征间的关系。采用Kaplan-Meier Plotter平台在线分析TMEM42表达与LSCC患者预后的关系。结果TGGA数据库在线分析发现503例LSCC组织中TMEM42表达较52例正常组织降低(P<0.05)。LSCC组织中TMEM42的累积光密度值低于癌旁组织(1.72±0.09 vs.3.83±0.61),mRNA水平少于癌旁组织(0.30±0.04 vs.1.06±0.06),TMEM42阳性率低于癌旁组织为[43.3%(26/60)vs.70.0%(42/60)],上述差异均有统计学意义(P<0.05)。TMEM42蛋白表达与LSCC年龄、性别、TNM分期和分化程度无关(P>0.05),而与淋巴结转移有关(P<0.05),其中TMEM42阳性者的淋巴结转移率低于阴性者[42.3%(11/26)vs.70.6%(24/34)]。TMEM42表达与LSCC患者的总生存期(OS)和疾病进展后生存期(PPS)有关,其中TMEM42高表达者的中位OS为67个月,优于低表达者的48个月(HR=0.79,95%CI:0.63~0.99,P=0.040),TMEM42高表达者的中位PPS为11个月,优于低表达者的6个月(HR=0.51,95%CI:0.28~0.92,P=0.023)。结论TMEM42在LSCC组织中低表达,且与淋巴结转移和不良预后有关,该因子有作为LSCC诊治靶点的潜能。Objective To investigate the expression of transmembrane protein 42(TMEM42)in lung squamous cell carcinoma(LSCC)tissues and its clinical significance.Methods TGGA database was used to analyze TMEM42 gene expression in LSCC tissues.Immunohistochemistry technology and real-time quantitative PCR were used to detect the expression levels of TMEM42 in 60 pairs of LSCC tissues and adjacent tissues.Relationship between TMEM42 expression and clinical pathological characteristics of LSCC was further analyzed.Kaplan-Meier Plotter platform was employed to analyze the relationship between TMEM42 expression and prognosis of LSCC patients online.Results Online analysis of TGGA database revealed that TMEM42 expression was lower in 503 LSCC tissues compared to 52 normal tissues(P<0.05).The integrated optical density of TMEM42 in LSCC tissues was lower than that in adjacent tissues(1.72±0.09 vs.3.83±0.61),and the mRNA level was lower than that in adjacent tissues(0.30±0.04 vs.1.06±0.06).The positivity rate of TMEM42 was lower in LSCC tissues than that in adjacent tissues[43.3%(26/60)vs.70.0%(42/60)],and the above differences were statistically significant(P<0.05).The expression of TMEM42 protein was not related to the age,gender,TNM stage,and differentiation degree of LSCC(P>0.05),but is related to lymph node metastasis(P<0.05).Among them,the lymph node metastasis rate of TMEM42 positive patients was lower than that of negative patients[42.3%(11/26)vs.70.6%(24/34)].TMEM42 expression was associated with overall survival(OS)and post-progression survival(PPS)in LSCC patients,with a median OS of 67 months for those with high TMEM42 expression,which was better than 48 months for those with low expression(HR=0.79,95%CI:0.63-0.99,P=0.040),and a median PPS of 11 months for those with high TMEM42 expression,which was better than 6 months for those with low expression(HR=0.51,95%CI:0.28-0.92,P=0.023).Conclusion TMEM42 is lowly expressed in LSCC tissues and is associated with lymph node metastasis and poor prognosis.This factor has the

关 键 词：肺鳞癌 跨膜蛋白42 临床意义 预后 

分 类 号：R734.2[医药卫生—肿瘤]