栽培条件对松杉灵芝多糖含量的影响及其抗氧化活性分析  

Effect of Cultivation Conditions on the Content of Polysaccharide in Ganoderma tsugae and its Antioxidant Activity

作　　者：张鑫 邬柯奕[2] 祝嗣臣 李玉 李兰洲[2] ZHANG Xin;WU Ke-yi;ZHU Si-chen;LI Yu;LI Lan-zhou(College of Horticulture,Shenyang Agricultural University,Shenyang 110866;Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi,Jilin Agricultural University,Changchun 130118;Heilongjiang Kunjian Agriculture Co.Ltd.,Qiqihaer 161200;National Center of Technology Innovation for Synthetic Biology,Tianjin 300308)

机构地区：[1]沈阳农业大学园艺学院,沈阳110866 [2]吉林农业大学食药用菌教育部工程研究中心,长春130118 [3]黑龙江坤健农业股份有限公司,齐齐哈尔161200 [4]中国科学院天津工业生物技术研究所,天津300308

出　　处：《生物技术通报》2024年第11期47-57,共11页Biotechnology Bulletin

基　　金：国家食用菌产业技术体系(CARS-20-17);天津市合成生物技术创新能力提升行动项目(TSBICIP-CXRC-006)。

摘　　要：【目的】优化松杉灵芝栽培和多糖提取条件,并分析其体外抗氧化活性,明确松杉灵芝多糖活性。【方法】以松杉灵芝的农艺性状、多糖含量为指标,优化松杉灵芝的栽培条件同时分析其体外抗氧化活性,进一步采用响应面设计法优化松杉灵芝多糖提取的方案,考察松杉灵芝粗多糖(GTP)对H_(2)O_(2)诱导的HepG2细胞的保护作用。【结果】松杉灵芝的最优栽培条件为遮阴率60%,温度30℃,湿度80%,CO_(2)浓度0.03%时,子实体质量和多糖含量均最高。松杉灵芝水提物表现出了良好的体外抗氧化活性,具有清除自由基和抗超氧阴离子作用。最佳提取方案为提取温度82℃、提取时间5.6 h、液料比56 mL/g时,得率为1.90%;GTP通过调控氧化应激相关蛋白的表达,保护H_(2)O_(2)诱导的HepG2细胞免受氧化损伤。【结论】明确了松杉灵芝的栽培条件及其多糖提取工艺,并证实其具有体外抗氧化活性,其对H_(2)O_(2)诱导的HepG2保护作用与氧化应激有关。【Objective】The aim of this study is to optimize the cultivation and polysaccharide extraction conditions of Ganoderma tsugae,analyze its antioxidant activity in vitro,and clarify the polysaccharide activity of G.tsugae.【Method】The agronomic traits and sugar content of G.tsugae were used as indicators to optimize the cultivation conditions and analyze its antioxidant activity in vitro.Furthermore,response surface design was used to optimize the extraction of G.tsugae crude polysaccharide(GTP)and to investigate its protective effect on H_(2)O_(2) induced HepG2 cells.【Result】The fruiting body mass and polysaccharide content are the highest,while the optimal cultivating conditions for G.tsugae is shading rate of 60%,temperature of 30℃,humidity of 80%,and CO_(2) concentration of 0.03%.Water extracts from G.tsugae also present promising antioxidant activity in vitro,with the ability to scavenge free radicals and resist superoxide anions.The optimal extraction method for sugar is at an extraction temperature of 82℃,extraction time of 5.6 h,and a liquid-solid ratio of 56 mL/g,reaching 1.90%.【Conclusion】The cultivation conditions and polysaccharide extraction process of G.tsugae were clarified,and it was confirmed that it has antioxidant activity in vitro.Its protective effect on H_(2)O_(2)-induced HepG2 is related to oxidative stress.

关 键 词：松杉灵芝 农艺性状 多糖 提取工艺 抗氧化活性 

分 类 号：S567.31[农业科学—中草药栽培]