狗尾草ARF基因家族的鉴定与表达分析  

Identification and Expression Analysis of ARF Gene Family in Setaria viridis

作　　者：王其昕 张凌馨 钟妮娜 熊王丹 WANG Qi-xin;ZHANG Ling-xin;ZHONG Ni-na;XIONG Wang-dan(College of Grassland Science,Qingdao Agricultural University,Qingdao 266109;Key Laboratory of National Forestry and Grassland Administration on Grassland Resources and Ecology in the Yellow River Delta,Qingdao 266109)

机构地区：[1]青岛农业大学草业学院,青岛266109 [2]黄河三角洲草地资源与生态国家林业和草原局重点实验室,青岛266109

出　　处：《生物技术通报》2024年第11期227-235,共9页Biotechnology Bulletin

基　　金：国家自然科学基金项目(31872879);山东省自然科学基金项目(ZR2023MC071);山东省大学生创新创业项目(S202310435231)。

摘　　要：【目的】生长素响应因子ARF(auxin response factor)可以调控生长素响应基因的表达,在植物生长发育中发挥着重要作用。从狗尾草全基因组范围内鉴定出SvARF基因家族成员,分析相关基因表达特征,为后续深入研究其与狗尾草生长发育的关系提供支撑。【方法】利用生物信息学方法从狗尾草基因组中鉴定了ARF转录因子成员,分析其系统进化、染色体定位和基因结构等情况。利用RT-qPCR(quantitative real-time polymerase chain reaction)分析该家族成员在生长素类除草剂2,4-D异辛酯处理后的表达响应情况。【结果】共鉴定到24个狗尾草SvARF基因家族成员,可分成4个亚家族,同一亚家族SvARF基因的结构相似且基因表达模式相近。不同SvARF基因的表达模式存在差异,且有组织特异性,III亚家族的SvARF基因在根茎分蘖处的表达量均较高。2,4-D异辛酯处理后,有9个SvARF基因的相对表达量发生显著变化,其中SvARF5、SvARF6、SvARF9、SvARF10、SvARF17和SvARF18表达量显著上调,SvARF14、SvARF22和SvARF24表达量显著下调。【结论】狗尾草SvARF基因家族有24个成员。生长素类除草剂影响SvARF基因的表达,暗示SvARF基因在抑制狗尾草植株分蘖中发挥重要作用。【Objective】The auxin response factor(ARF)plays an important role in plant growth and development through regulating the expression of auxin response genes.The SvARF gene family members were identified from the Setaria viridis genome,and the expression characteristics of related genes were analyzed,providing support for further research on the relationship between SvARF and the growth and development of S.viridis.【Method】The ARF transcription factors were identified from the S.viridis genome by bioinformatics.The phylogenetic evolution,chromosome location,and gene structure were also analyzed.Real-time quantitative polymerase chain reaction(RT-qPCR)were used to analyze the expression responses of SvARF gene to 2,4-D isooctyl esters.【Result】A total of 24 SvARF genes were identified.The members of SvARF gene family can be divided into 4 subfamilies.SvARF genes in the same subfamily shared similar expression pattern and genes in subfamily III expressed specifically in the tillers.After 2,4-D isooctyl ester treatment,the relative expressions of nine SvARF genes changed significantly.SvARF5,SvARF6,SvARF9,SvARF10,SvARF17 and SvARF18 were up-regulated,while SvARF14,SvARF22,and SvARF24 were down-regulated.【Conclusion】There are 24 members in the SvARF gene family.Auxin pesticide affects the expressions of SvARF genes,which may decrease the plant tiller number.

关 键 词：ARF转录因子 狗尾草 系统进化 结构特征 表达分析 分蘖 2 4-D异辛酯 

分 类 号：Q943.2[生物学—植物学]