连翘叶茶对大鼠肝硬化的影响及其机理研究  

Effect of Forsythia suspensa Leaf Tea on with Cirrhosis and Its Mechanism in Rats

作　　者：吴永娜 滕文龙 张磊[2] 王德富[1] 牛颜冰[1] WU Yong-na;TENG Wen-long;ZHANG Lei;WANG De-fu;NIU Yan-bing(College of Life Sciences,Shanxi Agricultural University,Jinzhong 030801;Department of General Surgery,The First Hospital of Lanzhou University,Lanzhou 730000)

机构地区：[1]山西农业大学生命科学学院,晋中030801 [2]兰州大学第一医院普外科,兰州730000

出　　处：《生物技术通报》2024年第11期285-295,共11页Biotechnology Bulletin

基　　金：财政部和农业农村部:国家现代农业产业技术体系(CARS-21);山西省自然科学基金(202203021212471);山西农业大学博士科研启动项目(J142202205)。

摘　　要：【目的】研究连翘叶茶粗提物及其茶水对四氯化碳(CCl4)诱导大鼠肝硬化的影响,阐明不同剂量连翘叶茶粗提物及茶水对肠道菌群多样性的影响及其机理。【方法】30只雄性SD大鼠按照平均体重相近的原则随机分为对照组、模型组、低、中、高剂量连翘叶茶灌胃组和茶水组,采用皮下注射CCl_(4)+灌胃CCl_(4)橄榄油混合液的方法构建肝硬化大鼠模型,建模成功后给予不同剂量连翘叶茶粗提物灌胃及茶水组干预10 d后,收取血清进行ELISA检测,肝脏组织通过HE染色和提取RNA进行RT-PCR检测,粪便样本进行16S rRNA测序。【结果】连翘叶茶粗提物及茶水组均能显著改善大鼠肝硬化形态,尤其茶水组可明显逆转肝硬化状态,肝细胞脂肪变性、中央静脉周围炎性细胞浸润和汇管区纤维结缔组织增生较模型组均明显改善;连翘叶茶粗提物及茶水组可显著提高益生菌Rothia、Parabacteroides和Lactobacillus丰度,促进不饱和脂肪酸合成和酸呼吸通路的富集;连翘叶茶粗提物及茶水组可抑制血液中DAO、TLR-4、LPS内毒素因子的水平,促进抑炎因子1L-4及肠道屏障功能因子ZO-1表达,各组之间肠道微生物组成与DAO、LPS、1L-4和TLR4相关,其中与肠道损伤指标DAO相关性最大。【结论】连翘叶茶粗提物及茶水组可能通过改善大鼠肝硬化形态、减弱炎症反应、改善肠道微生态功能失调及保护肠屏障功能抑制肝硬化发生。【Objective】To study the effects of crude extracts of Forsythia suspensa leaves and their tea solution on carbon tetrachloride(CCl4)induced liver cirrhosis in rats,and to elucidate the effects and mechanisms of different doses of the crude extracts and tea solution of F.suspensa leaves on gut microbiota diversity.【Method】The 30 male SD rats were randomly divided into control group,model group,low,medium,and high-dose F.suspensa leaf tea orally administered group,and tea water group according to the principle of similar average body weight.Liver cirrhosis rat model was constructed by subcutaneous injection of CCl_(4)+CCl_(4) olive oil mixture orally administered group.At the same time,different doses of F.suspensa leaf tea crude extract orally administered,and tea water group were intervened for 10 d.Serum was collected for ELISA detection,liver tissue was stained with HE,the liver tissue RNA was extracted for RT-PCR detection,and fecal samples were subjected to 16S rRNA sequencing.【Result】Both the crude extract of F.suspensa leaves and the tea group significantly improved the morphology of liver cirrhosis in rats,especially the tea group significantly reversed the status of liver cirrhosis.Liver cell steatosis,inflammatory cell infiltration around the central vein,and fibrous connective tissue proliferation in the portal area were significantly improved compared to the model group.The crude extract of F.suspensa leaves and the tea group significantly increased the abundance of probiotics Rothia,Paraacteroides,and Lactobacillus,promoting the synthesis of unsaturated fatty acids and the enrichment of acid respiration pathways.The crude extract of F.suspensa leaf tea and the tea group inhibited the levels of DAO,TLR-4,and LPS endotoxin factors in the blood,promoted the expression of antiinflammatory factor 1L-4 and intestinal barrier function factor ZO-1.The composition of intestinal microbiota among the groups was correlated with DAO,LPS,1L-4,and TLR4,with the highest correlation with intestinal injury i

关 键 词：连翘叶茶 肝硬化 微生物多样性 大鼠 炎症因子 机理 

分 类 号：R285.5[医药卫生—中药学]