癫痫发作后诱发炎症反应对癫痫大鼠学习记忆以及Erk1/2、p-Erk1/2和Caspase-3阳性表达的影响  

The Effect of Inducing Inflammatory Response after Epileptic Seizures on Learning and Memory as Well as the Positive Expression of Erk1/2,p-Erk1/2 and Caspase-3 in Epileptic Rats

作　　者：蔡海燕[1] 闫雨 焦旭文[2] 马江波[2] 丁银秀[2] CAI Haiyan;YAN Yu;JIAO Xuwen;MA Jiangbo;DING Yinxiu(Department of Neurology,People’s Hospital of Ningxia Hui Autonomous Region,Yinchuan 750002,China;Department of Human Anatomy,School of Basic Medical Sciences,Ningxia Medical University,Yinchuan 750004,China)

机构地区：[1]宁夏回族自治区人民医院神经内科,宁夏医科大学第三临床医学院,银川750002 [2]宁夏医科大学基础医学院人体解剖学系,银川750004

出　　处：《宁夏医科大学学报》2024年第11期1096-1101,共6页Journal of Ningxia Medical University

基　　金：宁夏自然科学基金项目(2022AAC03384,2022AAC03153)。

摘　　要：目的探究SD大鼠癫痫发作后不同时期海马细胞外信号调节激酶(Erk1/2)、磷酸化细胞外信号调节激酶(p-Erk1/2)和半胱氨酸天冬氨酸蛋白酶-3(Caspase-3)的表达变化情况。方法应用腹腔注射氯化锂-匹鲁卡品成功制备SD大鼠癫痫模型。分别通过定位巡航实验观察分析各组大鼠的学习记忆情况。应用Western blot法测定癫痫发作后各组大鼠海马Erk1/2、p-Erk1/2和Caspase-3的阳性表达情况。结果定位巡航实验结果显示,与对照(Con)组比较,SE组大鼠定位巡航所用时间和总航行路程较长(P均<0.05);Western blot结果显示,与Con组比较,SE组Tuj-1蛋白表达量减少(P均<0.001);SE 1 d组和7 d组GFAP和Iba1蛋白表达量增加(P均<0.01);SE 7 d组、14 d组和28 d组Erk1/2蛋白表达降低(P均<0.01);SE 1 d组和28 d组p-Erk1/2蛋白表达增加(P均<0.05);SE 7 d组、14 d组和28 d组Caspase-3蛋白表达水平上调(P均<0.05)。结论SD大鼠癫痫发作后诱发神经炎症反应,致大鼠学习记忆能力下降。且癫痫发作后Erk1/2信号通路被激活,星形胶质细胞异常增生,诱发了凋亡的级联反应,最终导致大量的神经元死亡,Caspase-3蛋白表达量明显上调。Objective To study the changes of hippocampal Erk1/2,p-Erk1/2 and Caspase-3 expressions in epileptic SD rats at different periods.Methods The epileptic rats’model was successfully induced by intraperitoneal injection of lithium chloride-pilocarpine.The learning and memory of the rats in each group were analyzed by positioning cruise experiment.The expression of Erk1/2,p-Erk1/2 and Caspase-3 proteins in each group were determined by Western blot analysis.Results The results of positioning cruise experiment showed that the epileptic rats were much longer in the time and the total distance of positioning cruise in epileptic rats(P all<0.05).Western blot analysis results showed that the protein level of Tuj-1 expression in epileptic rats was significantly decreased(P all<0.001).The protein levels of GFAP and Iba1 expression in SE 1 d and SE 7 d groups were evidently increased(P all<0.01).The expression levels of Erk1/2 proteins in the SE 7 d,14 d and SE 28 d groups were significantly decreased(P all<0.01).The expression levels of p-Erk1/2 proteins in the SE 1 d and SE 28 d groups were significantly increased(P all<0.05).The protein levels of Caspase-3 expression in each group were also markedly up-regulated(P all<0.05).Conclusion Neuroinflammatory response can be induced after epileptic seizure in SD rats,the learning memory abilities of epileptic rats were decreased.Erk1/2 signaling pathway is activated after epileptic seizure,which induced a cascade of apoptosis,astrocytes were abnormally activated,finally a large number of neurons died,and the expression of Caspase-3 protein was significantly up-regulated.

关 键 词：癫痫发作 神经胶质细胞 神经炎症 胶质细胞和神经元网络 

分 类 号：R742.1[医药卫生—神经病学与精神病学]