癌胚抗原联合外周血游离DNA中SEPT9基因甲基化检测在结直肠癌诊断中的应用价值  

Application Value of Carcinoembryonic Antigen Combined withSEPT9 Gene Methylation in Peripheral Blood Cell Free DNA Detection in Diagnosis of Colorectal Cancer

作　　者：陈征 马剑 刘天津 Chen Zheng;Ma Jian;Liu Tianjin(Fudan University,Shanghai 200433,China;Changzhou Cancer Hospital,Changzhou 213032,Jiangsu Province,China;Shanghai Genetalk Biotechnology Co.,Ltd,Shanghai 200435,China)

机构地区：[1]复旦大学,上海200433 [2]常州市肿瘤医院,江苏常州213032 [3]上海吉涛生物科技有限公司,上海200435

出　　处：《中国社区医师》2024年第33期119-121,共3页Chinese Community Doctors

摘　　要：目的:探究癌胚抗原(CEA)联合外周血游离DNA中隔膜蛋白9(SEPT9)基因甲基化检测在结直肠癌诊断中的应用价值。方法:选取2019年1月—2023年1月常州市肿瘤医院收治的154例疑似结直肠癌患者进行研究,根据病理检查结果分为腺瘤组(n=71)和结直肠癌组(n=83),选择同期100例健康体检者作为对照组,采集所有受试者静脉血,提取游离DNA,检测SEPT9基因甲基化、CEA水平,分析CEA联合外周血SEPT9基因甲基化检测诊断结直肠癌的效能。结果:结直肠癌组SEPT9基因甲基化阳性率高于腺瘤组和对照组(P<0.001)。结直肠癌不同分期患者SEPT9基因甲基化阳性率比较,Ⅳ期>Ⅲ期>Ⅱ期>Ⅰ期(P<0.001)。CEA阳性率比较:结直肠癌组>腺瘤组>对照组(P=0.007)。以病理检查结果为诊断“金标准”,CEA联合SEPT9基因甲基化检测诊断结直肠癌的效能较高(P=0.026)。结论:CEA联合外周血SEPT9基因甲基化检测诊断结直肠癌的效能较高,值得临床应用并予以推广,可为结直肠癌患者的早期诊断和治疗提供参考。Objective:To explore the application value of carcinoembryonic antigen(CEA)combined with Septin9(SEPT9)gene in peripheral blood cell free DNA methylation detection in the diagnosis of colorectal cancer.Methods:A total of 154 patients with suspected colorectal cancer admitted to Changzhou Cancer Hospital from January 2019 to January 2023 were selected for the study.They were divided into adenoma group(n=71)and colorectal cancer group(n=83)according to the pathological examination results,and 100 people who underwent physical examination during the same period were selected as the control group.The cell free DNA was extracted from the venous blood plasma.The SEPT9 gene methylation and CEA level were detected.The diagnostic efficiency of CEA combined with peripheral blood SEPT9 gene methylation detection for colorectal cancer was analyzed.Results:The positive rate of SEPT9 gene methylation in the colorectal cancer group was higher than that in the adenoma group and the control group(P<0.001).The positive rate of SEPT9 gene methylation in patients at different stages of colorectal cancer was compared and ranked as follows:stageⅣ>stageⅢ>stageⅡ>stageⅠ(P<0.001).The positive rate of CEA was compared and ranked as follows:colorectal cancer group>adenoma group>control group(P=0.007).Taking pathological examination results as the"gold standard"for diagnosis,CEA combined with SEPT9 gene methylation detection had a high diagnostic efficiency for colorectal cancer(P=0.026).Conclusion:CEA combined with peripheral blood SEPT9 gene methylation detection has a high diagnostic efficiency for colorectal cancer,it is worthy of clinical application and promotion,and can provide a reference for early diagnosis and treatment of colorectal cancer patients.

关 键 词：基因 SEPT9 DNA甲基化 结直肠癌 

分 类 号：R735.34[医药卫生—肿瘤]